How Cryostats Work & How We Save You Time & Money

Excedr - Cryostats & Microtomes

Regardless of type, the Excedr lease program can source all instrument types and accommodate any model preferences you might have. Request an estimate today and see how leasing a tissue sectioning instrument can benefit you and your laboratory.

All equipment brands/models are available

The Advantages of Excedr’s Cryostat Leasing Program:

  • Eliminates the upfront cost of purchasing equipment by spreading its cost over time
  • Minimizes equipment downtime with included complete repair coverage and preventive maintenance
  • Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
  • Expedites the administrative work needed for instrument procurement and logistics
  • Conserves working capital,  enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
  • Accommodates all manufacturer and model preferences

*Please consult your tax advisor to determine the full tax implications of leasing equipment.

three imaging screens showing different analysis results

A cryostat is a device commonly found in pathology, histology, histopathology, and research laboratories.

It is used to maintain low cryogenic temperatures for any devices, samples, and slides kept within it. Cryostats allow researchers or physicians to quickly section frozen tissues that can be observed and studied in microscopy applications.

Using this instrument, the physician can make a fast diagnosis, or a researcher can analyze tissue specimens more quickly and accelerate the development of a new treatment.

The method in which a cryostat’s low temperature is maintained can vary depending upon the make and model but often is done using a cryogenic fluid bath using liquid helium. Maintaining a cryogenic chamber temperature allows end-users to cut high-quality frozen sections for microscopic examination and study.

Types, Benefits, & Cryostat Machine Cost

a hand face up balancing a graduated cylinder on its index finger

Cryostats are incredibly important to anyone studying diseases, tissues, and the structure and function of these two topics. A typical device will function as follows:


A cryostat’s primary functions include maintaining cryogenic temperatures for any device or sample placed inside it and cutting histological slides with a cryostat microtome blade. The difference between a standard microtome and a cryostat is that the microtome cuts tissue sections at room temperature. In contrast, a cryostat containing a microtome keeps the temperature at a cryogenic level.

Furthermore, to ensure a tissue sample is sliced accurately, it’s also essential that the tissue is embedded. This is accomplished by placing it face up on a tissue holder and covering the sample with an embedding medium. The specimen holder, or chuck, is then set upon a freezing temperature bar.

In addition to cryogenic temperatures and embedding, disposable blades are provided by manufacturers, such as Leica, that help researchers and physicians consistently produce high-quality sections/slides. However, the type of blade you choose for your cryostat depends on the microtome and the material you’re sectioning. For general applications, you can choose between fixed or disposable blades. The blade itself goes in the blade clamp, which is one part of the microtome’s blade holder, located at the device’s front.

While frozen sections produced by cryosectioning are not as physically stable as paraffin-embedded sections, they provide excellent tissue sample preservation.

The section thickness can be as thin as 1 micrometer, as the micrometer’s cutting is exact. To prevent any curling or rolling while a lab technician slices a section, an anti-roll plate, a square or rectangular glass plate held inside a metal frame, is used to keep the sections flat.

The tissue section is then mounted to a glass slide within the device and dried and stained as needed. A standard method of staining frozen sections is by utilizing immunohistochemistry (IHC) staining. While the utility the machine offers can seem simple, the convenience that it adds is undeniable. In terms of temperatures, cryostats can maintain a specific degree that the tissue requires, which is generally somewhere between 20 and 30°C.

That all said, there are many types of cryostats available, so it’s essential to know which will fit your specific needs and requirements.


Bath Cryostats

The bath style is often similarly designed to vacuum flasks that utilize liquid helium. This includes having a cold plate placed in contact with the bath of liquid helium. The helium is replaced as it dissipates; this boil-off rate is determined by the size, style, frequency of use, and other factors.

The rapidity of the helium dissipating can be mitigated with the addition of vacuum shielding or the device having super-insulated walls. Most modern iterations are designed to include the super insulator materials as they are the most effective.


Continuous-Flow Cryostats

The continuous-flow models are cooled with liquid cryogens, almost exclusively liquid nitrogen or helium, from a storage dewar. Where the bath version has to refill the helium that dissipates manually, the boiled-off cryogen is continually replenished in a steady flow from the dewar.

The temperature controls of a continuous-flow cryostat system are done by controlling the rate at which the cryogen flows through the device, in addition to a heating wire that is part of a temperature control loop.


Closed-Cycle Cryostats

A closed-cycle type is differentiated by having a cryostat chamber through which cold helium vapor is pumped. This is attached to an external refrigerator that extracts the warmed helium exhaust vapor and then cools and recycles it. This type consumes a large amount of electricity to operate but never needs to be refilled with helium.


Multistage Cryostats

Sometimes it is necessary to have the cryostat be colder than liquid helium can accommodate.

This is done by adding additional cooler stages to the device, often a 1-K pot which allows the temperature to get as low as 1K. The only way to get below 1K with this device is to use magnetic refrigeration, which can be accomplished by adding another stage.


Biological Microtome Cryostats

These devices offer a wide variety of applications and come in many different types. Most of their applications boil-down to two different types of users: clinicians and researchers.

  • Clinical: If a surgeon needs a rapid intraoperative consultation, a cryostat can help get the immediate results needed by quickly, safely, and reliably slicing samples.
  • Research: Delaying critical research eventually hurts your bottom line. The utility and speed that a trusted cryostat can bring allow you to section more tissue and thus analyze more specimens in a single day.

Cryo Microtome Leases to Fit Every Need

an abstract scientific graph changing shapes

Whatever your lab equipment needs, we can help. At Excedr, we can lease cryostats or cryo-microtomes for all your electron microscopy needs, and our sales-leaseback option can help you free up your budget even further. 

Furthermore, if you require immunohistochemistry (IHC) stainers, we can help with that too. Call (510) 982-6552 or fill out our contact form to get in touch.

Operating Lease

This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional monetary savings.


If you recently bought equipment, Excedr can offer you cash for your device and convert your purchase into a long-term rental. This is called a sale-leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up money in your budget rather than tying it down to a fixed asset.

Cryostat Manufacturers & Models
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Leica Biosystems:
CM3050 S, CM1950, CM1860, CM1860 U, CM1520, CM3600 XP, AgProtect, CryoZone

Thermo Fisher:
HM525 NX, HM525 NX UV, HN520, HM550, CryoStar NX50, CryoStar NX70

Hacker Instruments & Industries:
Bright OTF5000 Microtome Cryostat, Bright 2212, Bright 8250, Bright 9400, H/I Cryo-Histomat MK-2, H/I Freezing Microtome, Clinicut

Bright Instruments:
Starlet 2212 Cyostat

Tanner Scientific:
Semi Automatic Cryostat, TNR TN50, TN50, TN2085, TN9000

Tissue-Tek Cryo3 Flex Cryostat


SLEE Medical:

Histo-Line Laboratories:
MC5050, MC4000, MC5000, MC3000

MEDITE Cancer Diagnostic:

Amos Scientific:
AST 550 and AST 500

Cool-Cut Automatic and Cool-Cut Semi-automatic

Lupetec Tecnologia Aplicada:

SM Scientific Instruments:
Microtome SMI-3010


Hubei Taiva Medical Technology Co., Ltd.:
TL-820B, TL-820A, TL-820C, TL-820C/D

Lab-Kits, Lab instruments & Facilities:
SF-2950, SF-2850

and more!


Operating Capital Benefits

Operating Capital Benefits

Excedr's operating lease structure allows you to keep your business credit line open for expansions, staffing, and other operational expenses. Additionally, it strengthens the cash flow of your business and keeps cash reserves free for business development opportunities.

Effects on Credit

Effects on Credit

Leasing / renting provides you with non-dilutive financing and does not hinder your future borrowing ability. You're able to acquire the equipment you need without the baggage associated with traditional financing.

Speed of Approval

Speed of Approval

Excedr's program allows you to respond quickly as your need for equipment and technology arises. You can be approved with minimal documentation and have the equipment you need in operation and generating revenue for your business quickly.

Refurbished Equipment

Refurbished Equipment

Unlike traditional financing and leasing companies, the Excedr program can accommodate refurbished equipment in addition to demo units. If you are looking for additional cost-savings, we recommend considering this option.