How A Mass Spectrometer Works & How Our Leases Save You Time & Money

Ionization is used to produce gas phase ions suitable for resolution in the mass analyzer. In order for a mass spectrometer to ionize a sample, or analyte, under analysis, an ion source is required. This ion source is how a mass spectrometer system generates ions. Afterward, the ions are transported via magnetic or electric fields directly to the mass analyzer.

Ionization techniques have played a vital role in determining what types of samples can be analyzed using MS. Depending on what you are analyzing, you’ll have to use specific types of ionization. Electric and chemical ionization are used for both vapors and gas, while matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization are used for solid or liquid biological samples.

There are two classifications of ionization used in MS.

The choice of ionization source is extremely important, and is usually classified as “hard” or “soft”.

Hard ionization fragments molecules, whereas soft offers little or no fragmentation. One example of hard ionization is electric ionization, or EI. This technique creates a high degree of fragmentation and yields in-depth mass spectra that can provide insightful, critical information. This type of ionizing tend to have lower m/z value compared to the molecular mass.

On the other hand, soft ionization includes processes such as chemical ionization (CI), electrospray ionization (ESI), and MALDI.

The mass analyzer is used for separating or resolving the ions according to their mass-to-charge ratio (m/z). This component is how the MS system separates ions.

There are six general types of analyzers used in MS:

• Time-of-flight (ToF)
• Quadrupole
• Ion trap
• Magnetic sector
• Electrostatic sector
• Ion cyclotron resonance

Each type has its advantages and disadvantages. While some offer high resolution and dynamic range, they can be expensive and bulky. Others may offer high sensitivity and reproducibility but lack the scan speed you require.

No single analyzer is suited for all analyses, and the advantages or limitations can have a significant impact on your lab. Therefore, understanding the different principles, features, and characteristics of each type is essential to choosing the one that best suits your needs and provides you optimal capabilities.

A key element of any system, the spectrometer’s detector is used to detect separated ions and convert them into a measurable signal. The separated ions are measured, and those measurements are sent to a data systems that creates a mass spectrum, a graphical representation of each ion’s m/z ratios and their relative abundance, plotted against their intensities.

There are several types of detectors available. The type you use depends on a variety of factors that can include spatial information retention and dynamic range, as well as spectrometer design and application requirements. They include:

• Photoplate detectors
• Faraday detectors (or Faraday cups)
• Electron multipliers (EM) or secondary electron multipliers (SEM)
• Daly detectors
• Image-current detection technology
• Focal-plane array detectors
• Cryodetectors

Many of the various types of detectors offer high sensitivity, as well as linear and quantitative response. However, some detectors are designed for very specific functions. Noise, uniformity, and counting single or multiple masses are also important considerations when choosing an instrument.

Ion detection technology has not received as much attention as the ion source and mass analyzer. Traditional analog (Faraday detectors)—and electron multiplier (EM) detectors have been used for decades, and although advances in ion detection technology have occurred, they have been incremental. Nonetheless, it is an area of importance, due to the fundamental need to detect ions.

A sample can be introduced into an MS system in several ways, depending on the experimental setup.

For many researchers and scientists in the life sciences, MS almost always means liquid-chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS), inductively coupled plasma-mass spectrometry (ICP-MS), as well as several other integrated analytical solutions.

Because mass spectrometers typically lack any analytical front end, integrating these systems with chromatographic technologies has become fundamental to analytical research. However, stand-alone devices can still be found in use for the analysis of pure solid and liquid compounds, or as chemical-specific analyzers that do not require separation from each other or the matrix they occur in.

Furthermore, tandem mass spectrometry, or MS/MS, is used to increase the overall ability of the two devices to analyze chemical samples. These coupled systems can be used alone as well, or together with GC or LC systems.

Mass spectrometry is used in a variety of applications across a wide range of fields. It’s applications commonly include:

• Proteomics: protein identification and characterization, peptide sequencing, and mapping numerous organelles.
• Metabolomics: metabolic fingerprinting and profiling, lipodomics studies, cancer screening and diagnosis, and biofuels generation and use.
• Pharmaceutical Analysis: drug testing and discovery, drug metabolism, drug degradation, product analysis, preclinical development, and pharmacokinetic and pharmacodynamic analyses.
• Clinical Diagnostics: therapeutic drug monitoring, biomarker discovery and profiling, disease detection and risk assessment, and direct analysis of biological samples.
• Forensic analysis: trace, fiber, and hair analysis, arson investigation, explosives and gun shot residue, and drug identification and abuse confirmation.
• Environmental Analysis: pesticide screening and quantitation, tap water testing, soil contamination analysis, and carbon dioxide and pollution monitoring.

Richard A. Yost and Christie G. Enke’s report on the triple quadrupole mass spectrometer in the Journal of the American Chemical Society in 1978 has been cited as the birth of mass spectrometer instruments.

However, the pair did not set out to create a mass spectrometer when they began to attempt entirely computerizing an analytical instrument. The triple quadrupole mass spectrometer, over 40 years old now, enabled scientists to use MS as an analytical chemistry technique. Before Enke and Yost’s work, MS was primarily used by organic and physical chemists. Since the pair’s report, the triple quadrupole mass spectrometer has turned into the workhorse of quantitative mass spectrometry.