Enzyme Linked-Immunosorbent Assay (ELISA)

the four steps of an enzyme-linked immunosorbent assay (ELISA)

Overview

ELISA, or enzyme-linked immunosorbent assay, is a plate-based assay technique used in analytical biochemistry and other fields of study to detect and quantify soluble substances, such as proteins, antibodies, and peptides in complex mixtures.

Originally described by Engvall and Perlmann in 1971,  ELISA enables the analysis of protein and other samples, which have been immobilized in microplate wells using specific antibodies. Immunosorbent assays are typically performed in 96-well or 384-well polystyrene plates that passively bind antibodies and proteins, are simple to design, and easy to perform.

Using reagents to bind and immobilize the proteins to the microplate surface makes the separation of bound and unbound material during the assay much simpler than other separation methods.

Resources

Enzyme-Linked Immunosorbent Assay: Overview & Application

Enzyme-Linked Immunosorbent Assay: Overview & Application

Enzyme-Linked Immunosorbent Assay: Overview Enzyme-linked immunosorbent assay, or ELISA, is a plate-based biochemical assay used in labs to detect and quantify substances in a complex mixture, such as…
Cell Lysate: Overview & Applications

Cell Lysate: Overview & Applications

Overview: Cell Lysate Cell lysate is the preparation obtained after lysing a cell population in labs using certain chemical reagents and enzymes, or by osmotic and mechanical disruption.  They ca…
GST Tag: Overview & Definition

GST Tag: Overview & Definition

Definition: GST-tagged Proteins Protein affinity tags are special proteins or peptides added to the C- or N-terminus of recombinant proteins for their easy and targeted purification. Many protein affi…
Wash Buffer: Definition & Overview

Wash Buffer: Definition & Overview

Definition: Wash Buffer Lab experiments need to be carefully performed in order to achieve reliable and accurate results. Especially when it comes to high-throughput assays that require quality and ex…
His-Tag: Definition & Overview

His-Tag: Definition & Overview

Definition: His-Tag Affinity tags are powerful tools used in life sciences labs for basic biological studies as well as structural and functional proteomics.  The tags are structurally a short se…

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