How Raman Spectrometers Work & How We Save You Time & Money
Excedr’s lease program can source all instrument types and accommodate any brand preferences you may have. Request a Raman spectrometer estimate today and see how leasing can significantly decrease your instrument’s upfront price.
All equipment brands/models are available
The Advantages of Excedr’s Raman Spectrometer Leasing Program:
- Eliminates the upfront cost of purchasing equipment by spreading its cost over time
- Minimizes equipment downtime with included complete repair coverage and preventive maintenance
- Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
- Expedites the administrative work needed for instrument procurement and logistics
- Conserves working capital, enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
- Accommodates all manufacturer and model preferences
*Please consult your tax advisor to determine the full tax implications of leasing equipment.
Raman spectrometers employ Raman spectroscopy, a nondestructive chemical analysis technique that provides detailed information about a sample’s chemical composition, molecular interactions, crystallinity, and phase and polymorphy.
Specifically, the Raman effect is a light scattering technique based on the principle that molecules scatter incident light from a highly intense light source, typically a laser source. The scattered, incidental light is usually the same wavelength as the laser light and does not provide essential data about the sample. That’s because when light hits something, most of the photons that are scattered are the same energy as the light it was hit with. This scattered light is also referred to as Rayleigh Scatter.
However, a minimal amount of light is scattered at a different wavelength than the Rayleigh Scatter and is indicative of an analyte’s chemical structure. This shorter wavelength scattered light is called Raman Scatter, or Raman effect. It is named after Sir C.V. Raman.
In other words, Raman spectroscopy is a type of molecular spectroscopy that relies on inelastic scattering (Raman Scatter) of light to detect vibrational, rotational, and other low-frequency modes in a sample.
It is used in chemistry to identify molecules by providing a structural fingerprint, represented by a Raman spectrum. This spectrum is characterized by several peaks that describe the intensity and wavelength position of the scattered light from a sample and are unique to specific molecules and materials. Each peak relates to a particular molecular bond vibration, including both individual bonds and groups of bonds.
Raman spectral libraries are often used to identify a sample based on its Raman spectrum. The libraries contain thousands of samples’ Raman spectra, collected from various positions of each sample.
Because Raman looks at the scattering of light rather than its absorption, the sample preparation is much less complex, and there are no aqueous absorption bands to throw off the data. When a monochromatic laser interacts with a sample, the light will scatter. Furthermore, these devices offer both low/medium resolution and high-resolution capabilities.
Raman Spectrometer Techniques, Methods, & Costs
These devices’ instrumentation varies depending on the experimental capabilities you wish to have. We’ll review the basics. Furthermore, Raman spectrometers rely on different techniques to observe change, which will also be explained in further detail below. We’ll cover some of the most common ones, as several different types of Raman spectroscopy are being used today.
Raman spectrometers are made of three main components:
- Sampling interface
The laser’s excitation wavelength can vary depending on the sample being analyzed using Raman spectroscopy. The various wavelengths include some typical examples: ultraviolet, visible, and near-infrared (NIR). The one you choose will depend on your goals and the experimental capabilities you wish to have.
In many spectrometers today, the sampling interface is a type of fiber-optic probe. It can be augmented to fit a range of optical microscopes, liquid flow cells, gas flow cells, and various sampling chambers.
The spectrometer provides high resolution and low power consumption without much noise and includes the detector. The detector type depends on the laser source being used. When visible light is used, a standard charge-coupled device (CCD) detector is utilized; however, various CCDs exist that are optimized for specific wavelengths. In the case of UV excitation, a particular CCD detector is used, along with objective lenses and diffraction gratings.
Surface-Enhanced Raman Spectroscopy (SERS)
A highly accurate and powerful technique, surface-enhanced Raman spectroscopy (SERS) improves normal Raman scattering by huge orders of magnitude and can be used to detect single molecules.
This technique applies Raman intensity and improves Raman scattering through an electromagnetic amplification mechanism. The enhancement occurs on the surface of a rough metal substance or via nanostructures consisting of magnetic-plasmonic silica nanotubes.
Due to its ability to identify chemical species and analyze the makeup of a mixture on the nanoscale, this technique is used extensively in chemistry, pharmaceuticals, and materials science.
Coherent Anti-Stokes Raman
Coherent anti-Stokes Raman Spectroscopy (CARS), also known as coherent anti-stokes Raman scattering, is similar to conventional Raman spectroscopy. It is also used to measure the vibrational signatures of molecules.
However, it differs from the other technique because it employs multiple photons to address the vibrations. Doing so produces a coherent signal or identical wave sources. This makes CARS much stronger than processes like spontaneous Raman emission.
Fourier Transform (FT) Raman
Fourier Transform (FT) Raman spectroscopy relies on a specific type of configuration, which is designed to collect wavelength-stable and fluorescence-free measurements. A conventional FT Raman spectrometer comprises an excitation laser source, a sampling interface, and an interferometer.
The use of an interferometer makes FT Raman distinct from dispersive techniques, such as Dispersive Raman, which uses a diffraction grating spectrometer to disperse the light scattered from a sample. While the diffraction grating will detect the scatter via a CCD detector, producing a Raman spectrum directly, the FT Raman spectrometer’s interferometer will introduce a path between the light source and the signal beams, creating an interference pattern. This pattern is used to reconstruct the Raman spectrum.
By using a specific wavelength to cause scattering, resonance Raman spectroscopy can increase the intensity of Raman scattering. This is done by choosing a wavelength that either overlaps or is extremely close to the electronic transition of the sample that is being observed.
Due to resonance Raman spectroscopy’s increased intensity, it can detect samples with extremely low concentrations in a substance. One major disadvantage of this technique is that the fluorescence of an object may throw off the data collected and should be accounted for. This makes it an instrumental technique in analyzing environmental pollutants that have concentrations in the parts per billion range.
By shining light through a sample in the direction of the excitation laser, transmission Raman spectroscopy allows for bulk analysis of powders, tablets, and opaque substances.
By shooting the light through the object and analyzing the light that comes out the other side, it allows for analysis of the entire volume of the material.
Their ability to perform fast, quantitative analysis of substances makes them useful in pharmaceutical and medical analysis, as well as material sciences.
Raman Optical Activity
Also known as spontaneous vibrational Raman optical activity scattering, this vibrational spectroscopy technique looks at the difference in intensity of Raman scattered from the right and left circularly polarized light.
Similar to vibrational circular dichroism, Raman optical activity directly looks at chirality, or molecular vibrations. Due to its ability to observe chirality, this spectroscopic technique is very useful in chemistry and biology.
Raman spectroscopy can be used in microscopic analysis, with a spatial resolution in the order of 0.5-1 micrometer (µm) using a Raman microscope. A Raman microscope combines a Raman spectrometer with a standard optical microscope and enables high-magnification visualization of a sample and Raman analysis using a microscopic laser spot.
The data collected can be quantitative or qualitative and is most suitable for the quick characterization of chemical compositions while providing a visual image of the sample.
Sir Raman & Raman Scattering
Sir Chandrasekhara Venkata Raman, or C.V. Raman, was a professor at Calcutta University. In 1930 he won the Nobel Prize for Physics for his work observing light scattering. His and his team’s discovery would later be named after C.V. Raman and be known as Raman scattering.
Sir Raman famously found his inspiration in light scattering while traveling from London to Bombay. He became fascinated with the blue color of the Mediterranean sea and was not satisfied with the current explanation for this phenomenon at the time. He would find that the color of the sea was due to the scattering of light by water molecules.
His studies of scattered light continued and led him to find that a small amount of light scattered was of a different color than the incident light. He describes this phenomenon in his published report in Nature, titled “A New Type of Secondary Radiation,” and in 1930, this discovery would win him the Nobel Prize for physics.
High-Throughput Screening Raman Spectrometer Leases to Fit Every Need
Scientists and researchers rely on high-performance Raman spectroscopy for identifying molecules and observing the vibrational behavior of substances. However, purchasing a spectrometer outright can be difficult or even detrimental operationally. This is due to cutting-edge technology being costly.
In fact, we’ve seen laboratories hamstring their operations by taking on the high upfront costs. Instead of trying to take the usual equipment procurement route, consider leasing. Our program is designed to provide access to the technology you need to complete your work and make asset and cash flow management simpler.
So whatever your Raman needs, be it Raman spectroscopy or Raman microscopy, we can help. Furthermore, our spectrometer and Raman microscope leases are brand agnostic, meaning you can work with any vendor you’d like. With the money you reserve, you’ll be in a position to succeed and grow.
Let us know if you need other spectrometers, such as an FTIR or X-ray spectrometer. Our selection of lab equipment is comprehensive and brand agnostic. If you already have an instrument quote from a manufacturer, feel free to request a lease estimate. We can create customized terms just for you! Alternatively, if you’d like to learn more about our program, get in touch above, and we can discuss your leasing options in detail.
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional monetary savings.
If you recently bought equipment, Excedr can offer you cash for your device and convert your purchase into a long-term rental. This is called a sale leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up money in your budget rather than tying it down to a fixed asset.
Raman Spectrometer Manufacturers & Models
Mira P Advanced, Mira P Basic, Mira DS Basic, Mira DS Advanced, Mira M-1, Mira M-3, SPELEC RAMAN Instrument
DXR3 SmartRaman, iXR Raman Spectrometer, TruScan RM Handheld Raman Analyzer, FirstDefender RM Chemical Identification System, FirstDefender RMX Handheld Chemical Identification
Cobalt Insight100 Series, Cobalt Insight200M, TRS100, RapID System, Resolve
NRS-5000 Series, NRS-5100, NRS-5200, NRS-5500, NRS-5600, NRS-7000 Series, NRS-7100, NRS-7200, NRS-7500, NRS-7600, RMP 500 Series, RFT-6000 FT Raman Spectrometer
MacroRAM, Modular Raman Spectrometer, iHR320, iHR550, FHR1000, THz-Raman, LabRAM Odyssey, LabRAM HR, LabRAM HR Evolution,
NanoRam, TacticID-GP, TacticID-N, TacticID-1064, i-Raman Prime, i-Raman Plus, i-Raman EX, STRam, QTRam,i-Raman Pro
MultiSpec Raman Spectrometer
Raman All-In-One (AIO), Raman Walkup All-In-One (WAIO), TouchRaman Immersion technology, TouchRaman BallProbe
ChemLogix,ChemLogix GasRaman, GasRaman NOCH-1, GasRaman Noch-2, Assurx, Assurx-G7, ExpertRaman W-Series
Cora 7X00 series, Cora 100, Cora 5X00 series
HyperFlux PRO plus
Shakti PR-1000 Raman Analyzer, Portable Raman Systems
Raman-HR-TEC-405, Raman-ER-TEC-405, Raman-HR-TEC-532, Raman-ER-TEC-532, Raman-HR-TEC-785, Raman-SR-785, Raman-HR-TEC-1064
CHEM 500, Inspector 500, Inspector 300, Inspector Scope, ReporteR, Advantage Series Benchtop Raman, Advantage 532, Advantage 633, Advantage 785, Advantage 1064, SERS
RaPort, R532, R532-50, R1064, R1064-1c
Morphologi 4-ID, Morphologi G3-ID
NS2 NanoSpectralyzer, NS3 NanoSpectralyzer
Identity Raman Reader
AvaRaman System, AvaRaman-532 TEC, AvaRaman-532 HERO-EVO, AvaRaman-785 TEC, AvaRaman Bundles, AvaRaman-A, AvaRaman-B, AvaRaman-D
Advanced Integrated Scanning Tools for Nano Technology:
QE Pro-Raman, QE Pro (Custom)
Applied Instrument Technologies:
RPM View, RPM 785, RPM MD Series
Angstrom Advanced Inc.:
RM-1000, RM-2000, RM-5000
Raman Spectrometer, Raman-SR, Raman-HR, Raman-HR-TEC, Raman-SR-TEC-IG, Raman-HR-TEC-IG
RA802 Pharmaceutical Analyzer, RA816 Biological Analyzer
J&M Analytic AG:
TIDAS L 1100 Raman, TIDAS S1200R-Raman