PCR, qPCR, & DNA Amplification

the front of a PCR machine displayed next to PCR consumables, RNA template, and PCR tube

Polymerase Chain Reaction: Definitions & Consumables

Since its introduction, PCR, short for polymerase chain reaction, has contributed greatly to innovations in biopharmaceuticals, biotech crops, and industrial biotechnology. The development of hundreds of technologies in the aforementioned areas, such as genetic cloning, forensics, and genetic analysis, is the result of PCR.

The technique, sometimes referred to as molecular photocopying, is a rapid and inexpensive in vitro technique used to amplify copies of small segments of DNA. This process can produce billions of copies quickly and efficiently.

PCR has several applications in molecular biology and beyond, including:

  • Selective DNA Isolation
  • Medical and Diagnostic uses
  • Genetic research
  • DNA Fingerprinting
  • Food Safety

PCR assays are also used in the detection, diagnostic analysis, and sequencing of viral genomes. A variant of PCR (RT-PCR) has been extensively used to test patients for COVID-19 infections from the SARS-CoV-2 viral genome.

There are several types of PCR used today, including:

  • qPCR
  • RT-qPCR
  • dPCR
  • ddPCR
  • Hot-start PCR
  • High fidelity PCR
  • Multiplex PCR
  • Microfluidic PCR

Learn more about PCR, how it works, and what consumables are required to perform DNA amplification.

PCR Posts

Deoxynucleotide Triphosphates (dNTP): Components & Overview

What Is dNTP? Nucleoside triphosphate is a biomolecule involved in building nucleic acids (DNA and RNA). Structurally, they contain nitrogenous bases bound to a ribose sugar and three phosphates. And,…

PCR Master Mix: Definition, Types, and More

What is a Real-time PCR Master Mix? PCR master mix, as the name suggests, is a premixed solution of all reagents and essential components required to run a PCR assay. It contains dNTPs (dATP, dGTP, dT…

What is the Role of MgCl2 in PCR?

Polymerase Chain Reaction (PCR) Basics Did you know Mullis and Michael Smith won the Nobel Prize for discovering the Polymerase Chain Reaction (PCR) in 1983? This groundbreaking technology-enabled sci…

What is Deoxyguanosine Triphosphate (dGTP)?

What is dGTP? Deoxyguanosine triphosphate (dGTP) is a nucleotide with a purine base (guanine), deoxyribose sugar, and three phosphate groups. It’s one of the four dNTPs (deoxynucleoside triphosphate) …

What is Deoxythymidine Triphosphate (dTTP)?

What is dTTP? 2′-deoxythymidine 5′-triphosphate (or dTTP) is one of the nucleotide bases among three other dNTPs (deoxynucleoside triphosphate)—dATP, dGTP, and dCTP—that are involved in th…

What is dCTP? Definition & Use Cases

What is Deoxycytidine Triphosphate (dCTP)?  2′-deoxycytidine 5′-triphosphate (or dCTP) is a nucleoside triphosphate (a nucleotide), containing cytosine as its pyrimidine base. It also…

The Basics of Reverse Transcription PCR (RT-PCR)

What is Reverse Transcription Polymerase Chain Reaction (RT-PCR)? Reverse transcription-polymerase chain reaction is a molecular technique commonly called RT-PCR. PCR stands for polymerase chain react…

dATP: What is Deoxyadenosine Triphosphate?

What is dATP? Deoxyadenosine triphosphate (dATP) is a nucleotide that is used as a building block in DNA synthesis, during both in vivo replication and transcription and in vitro replication technique…

DNA Polymerase: What Is It and What Does It Do?

What is DNA Polymerase? A number of molecular biologists originally believed that the first DNA molecule is the reason for life on earth. That sentiment changed after many years of research and experi…

What is Polymerase Chain Reaction (PCR)?

Effective research and advancements in life science would not be possible without the discovery of PCR. PCR has contributed greatly to innovations in biopharmaceuticals, biotech crops, and industrial …