PCR & DNA Amplification

PCR is a powerful DNA amplification technique central to modern molecular biology. Learn how it supports research, diagnostics, and biotech breakthroughs across life sciences.

A game that looks similar to Tetris. It represents dNTP, a biomolecule used to build nucleic acid.
April 20, 2022
 
PCR & DNA Amplification
Deoxynucleotide Triphosphates (dNTP): Definition & Overview

What Is dNTP? Nucleoside triphosphate is a biomolecule involved in building nucleic acids (DNA and RNA). Structurally, they contain nitrogenous bases bound to a ribose sugar and three phosphates. And,...

A puzzle that forms the shape of a DNA strand. Two pieces of the puzzle have been pulled out.
February 15, 2022
 
PCR & DNA Amplification
What Is Deoxythymidine Triphosphate (dTTP)?

What is dTTP? 2′-deoxythymidine 5′-triphosphate (or dTTP) is one of the nucleotide bases among three other dNTPs (deoxynucleoside triphosphate)—dATP, dGTP, and dCTP—that are involved in th...

A textbook with pages of formulas. A few of the formulas are highlighted.
February 15, 2022
 
PCR & DNA Amplification
What Is dCTP? Definition & Use Cases

What is Deoxycytidine Triphosphate (dCTP)? 2′-deoxycytidine 5′-triphosphate (or dCTP) is a nucleoside triphosphate (a nucleotide), containing cytosine as its pyrimidine base. It also...

A row of rain falling down on an umbrella.
February 15, 2022
 
PCR & DNA Amplification
What Is the Role of MgCl2 in PCR Amplification Reactions?

Polymerase Chain Reaction (PCR) Basics Did you know Mullis and Michael Smith won the Nobel Prize for discovering the Polymerase Chain Reaction (PCR) in 1983? This groundbreaking technology-enabled sci...

Four test tubes in a row. Each test tube has different liquid and sedimentation inside.
February 15, 2022
 
PCR & DNA Amplification
PCR Master Mix: Definition, Types, & More

What is a Real-time PCR Master Mix? PCR master mix, as the name suggests, is a premixed solution of all reagents and essential components required to run a PCR assay. It contains dNTPs (dATP, dGTP, dT...

Four cogs inside a machine. One cog is blue. A red line runs through the center of the drawing.
February 15, 2022
 
PCR & DNA Amplification
What Is Deoxyguanosine Triphosphate (dGTP)?

What is dGTP? Deoxyguanosine triphosphate (dGTP) is a nucleotide with a purine base (guanine), deoxyribose sugar, and three phosphate groups. It’s one of the four dNTPs (deoxynucleoside triphosphate) ...