Learn how ELISA assays are used to detect and quantify proteins, antibodies, and peptides in complex samples. Topics include assay formats, optimization tips, and applications in life sciences and clinical diagnostics.
ELISA blocking buffers prevent non-specific binding, ensuring accurate results. They improve signal-to-noise ratio and are crucial for ELISA assays. Learn how to choose the right blocking buffer to optimize your immunoassays.
Explore the ins and outs of ELISA detection. In this blog post, we review what is ELISA, ELISA detection, the types of ELISA, and methods of detecting the presence of an antigen or antibody. Read on to learn more.
ELISA: Overview Enzyme-linked immunosorbent assay (ELISA) antibodies are crucial elements of the ELISA assay that are used to study proteins. Before proceeding to understand the antibody roles, it’s e…
Enzyme-Linked Immunosorbent Assay: Overview Enzyme-linked immunosorbent assay, or ELISA, is a plate-based biochemical assay used in labs to detect and quantify substances in a complex mixture, such as...
Cell lysate is the preparation obtained after lysing a cell population in labs using certain chemical reagents and enzymes, or by osmotic and mechanical disruption. They ca...
Definition: GST-tagged Proteins Protein affinity tags are special proteins or peptides added to the C- or N-terminus of recombinant proteins for their easy and targeted purification. Many protein affi...