How Confocal Raman Microscopy Works & How We Save You Time & Money

Excedr - Raman Microscopes

Excedr can source all equipment types and accommodate any model preferences your lab might have. Request a confocal Raman microscope quote today and see how leasing can benefit your laboratory.

All equipment brands/models are available

The Advantages of Excedr’s Raman Microscope Leasing Program:

  • Eliminates the upfront cost of purchasing equipment by spreading its cost over time
  • Minimizes equipment downtime with included complete repair coverage and preventive maintenance
  • Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
  • Expedites the administrative work needed for instrument procurement and logistics
  • Conserves working capital,  enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
  • Accommodates all manufacturer and model preferences

*Please consult your tax advisor to determine the full tax implications of leasing equipment.

a microscope, an ocular lens, and microscopic image next to each other

Raman microscopy, also referred to as confocal Raman microscopy, is a technique that combines Raman spectroscopy with optical microscopy.

By combining an optical microscope with a Raman spectrometer, scientists and researchers can generate high-resolution images of microscopic samples. The optical microscope (typically a confocal microscope) provides spatial resolution and filtering, while the spectrometer provides spectral information. For this reason, it is a superior technology of choice for the characterization of various materials and samples.

The optics enable the analysis volume within the sample to be spatially filtered with high-resolution across all axes. The spectrometer—sensitive to the vibrational modes of the sample—provides chemical, physical, and structural insights. 

When these spatial filters are added to a Raman microscope, it is called a confocal Raman microscope. In confocal Raman microscopy, the resolution is so fine that individual particles as small as 1 micron or less can be analyzed. The resolution of these microscopes is also affected by the specific wavelengths of the laser and the type of objective lens used.

Raman microscopes are widely used in materials science, cell biology, and medicine and have applications in pharmaceutical development, particulate identification, chemical imaging and analysis, and molecular and polymer analysis. In addition, these devices can be used to map areas of a sample or perform depth profiling without sample preparation. 

One of the reasons these microscopes are considered so helpful is that, by taking Raman spectrums at all points on an object and filtering for specific spectral bands, an image can be reconstructed from the spectral information. This ability to analyze and produce an image of an object from its Raman spectrum distinguishes Raman microscopy from Raman spectroscopy.

Raman Microscope Basics, Components Imaging Techniques

a hand holding an ocular lens

We’ll briefly explain the fundamentals, components, and various imaging techniques of confocal Raman microscopy, a technique made possible through the combination of confocal microscopy and Raman spectroscopy.

It’s important to note that Raman spectrometers are generally similar to Raman microscopes when they are equipped with optical components. (This makes distinguishing between the two a little confusing. Just remember that when a device can both analyze a sample and produce an image of the sample, it’s often considered a Raman Microscope.)

Raman Microscopy Basics

Raman spectroscopy (the basis for Raman analysis in this type of microscopy) is based on the Raman effect, an inelastic scattering phenomenon. It’s a type of vibrational spectroscopy used to analyze a sample chemically. An analysis is performed using a monochromatic light (i.e., a laser), which acts as an excitation source and creates a molecular vibration.

When the light interacts with the sample, a small part of it changes wavelengths. This change is referred to as the Raman effect, and the altered light is collected to gain chemical insights about the sample.

In contrast to optical techniques like infrared microscopy, Raman microscopes use light that is compatible with simple glass optics. This means that Raman microscopes are often developed based on a very high-quality optical microscope.

Generally speaking, no elaborate sample preparations are necessary when using Raman microscopy. Instead, the samples are placed beneath the microscopy as they are. When sample preparation is, in fact, critical, simple cross sections are prepared, or large workpieces are created to fit on the stage.

However, the same sample restrictions required in Raman spectroscopy still apply here, and the sample may not show strong fluorescence or absorbance of the excitation wavelength.

For accurate and reproducible results, calibration of the wavelength axis is necessary. Whether minor or major, many consequences usually happen in terms of wavenumber calibration. For this reason, calibration and recalibration is performed regularly.

To calibrate or recalibrate a device, a silicon standard is measured. However, modern microscopes typically offer continuous calibration for ease of use and reduction of manual recalibration.


Spectral Resolution

Spectral resolution describes the ability to resolve spectral features into their individual elements. If it is too small, spectral signals can disappear in wide “bands.”

On the other hand, if the resolution is too large, the measurements take much longer than required, without any advantages for the end-user. This highlights the importance of knowing which spectral resolution is ideal for a particular sample. What makes the resolution “too low” or “too high” depends on the respective application and the analytical task at hand.


Spatial Resolution

Spatial resolution is important because it influences how sharply we see objects. In Raman microscopy, it is vital to distinguish different structures in a sample. The better the spatial resolution, the more detailed the information obtained.

Various parameters determine the lateral and axial resolution. A confocal Raman microscope must be used to achieve the highest resolution in both areas. Typically, spatial resolution is a decisive parameter in Raman imaging.


Major Components

Despite the variations, Raman microscopes and spectrometers typically comprise the following components:

  • Optical microscope: contains all the standard structural components of a microscope. This includes eyepiece (ocular lens), objective turret, objective lens, focus knobs, light source, condenser, and stage/mechanical stage.
  • Raman spectrometer: this includes an excitation source (i.e., excitation laser), laser rejection filters for collecting Raman scattering light, diffraction grating that bends the Raman shifted light according to wavelength, and the spectrometer itself (which acts as the detector and sends a signal and passes it to a computer system for decoding.)
  • Optical sensitive detector: Raman systems come equipped with or modified to include an optical sensitive detector device. This includes charge-coupled devices (CCDs) or photomultiplier tubes (PMTs).

When selecting a Raman device, it’s essential to consider all the various components that make up the microscope and spectrometer and their optimal configuration for a particular application. These factors will affect your ability to perform research accurately and efficiently.


Raman Imaging

Generating images of chemicals pose interesting problems due to the size of the sample that is being imaged and what scientists wish to display.

Raman imaging is a technique that uses Raman spectroscopy to develop chemical images by producing false colors representing the material’s chemical makeup. After full Raman spectra of the target, microscopic regions are obtained, specific colors are used to represent the material’s composition, phase, crystallinity, and strain at that pixel.

Other imaging techniques used include direct imaging, hyper-spectral imaging, and correlative imaging.


Correlative Raman Imaging

This type of imaging refers to a group of techniques that combine Raman with other imaging methods. Popular correlative techniques include Raman-SEM, Raman-AFM, and Raman-SNOM.


Confocal Raman Imaging Microscope Leases to Fit Every Need

an ocular lens and eyepiece tube with a microscopic image displayed

Raman microscopes are powerful imaging and analysis tools that many laboratories need to perform a wide range of analyses. However, buying one outright can prove to be financially challenging. The costs of a Raman device can be high, and procuring one the traditional way as a lab with a tight budget can seem impossible.

This inaccessibility includes many of the cutting-edge instruments and systems used today. Being a lab without a massive funding round backing you can be challenging.

Our lease program is a creative solution to a common problem in the life sciences industry today: inadequate funds. You can simplify equipment procurement and cash management when leasing with us, making operations more robust and flexible. Furthermore, our selection of equipment is comprehensive and brand agnostic. So whatever your microscopy needs—Raman, confocal, or electron—we can help.

If you have a confocal Raman microscope quote from a manufacturer already, let us know by requesting a lease estimate below! We can use it to create a customized lease just for you, which determines how much your payments will be each month during the lease term.

Or, if you just want to learn more, get in touch with us above. Fill out our contact form online. We can discuss your specific needs in further detail.

Operating Lease

This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional monetary savings.


If you recently bought equipment, Excedr can offer you cash for your device and convert your purchase into a long-term rental. This is called a sale leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up money in your budget rather than tying it down to a fixed asset.

Confocal Raman Microscope Manufacturers & Models
Learn More

DXR2xi, DXR 2 microscope


LabRAM HR Evolution, XploRA PLUS, XploRA INV, XploRA ONE


NRS Series, NRS-4500 Series, NRS-5000 Series, NRS-7000 Series

inVia, inVia Qontor

Photon etc:

alpha300 R, access, apyron, alpha300 RI

NT-MDT Spectrum Instruments NTEGRA SPECTRA II

Apollo II

and more!


Operating Capital Benefits

Operating Capital Benefits

Excedr's operating lease structure allows you to keep your business credit line open for expansions, staffing, and other operational expenses. Additionally, it strengthens the cash flow of your business and keeps cash reserves free for business development opportunities.

Effects on Credit

Effects on Credit

Leasing / renting provides you with non-dilutive financing and does not hinder your future borrowing ability. You're able to acquire the equipment you need without the baggage associated with traditional financing.

Speed of Approval

Speed of Approval

Excedr's program allows you to respond quickly as your need for equipment and technology arises. You can be approved with minimal documentation and have the equipment you need in operation and generating revenue for your business quickly.

Refurbished Equipment

Refurbished Equipment

Unlike traditional financing and leasing companies, the Excedr program can accommodate refurbished equipment in addition to demo units. If you are looking for additional cost-savings, we recommend considering this option.