How a Two Photon Microscope Works & How Leasing Benefits Your Lab
Even with the many instrument variations and types, Excedr’s leasing program can accommodate any preferences your lab may have regarding brand or model. Get in touch with us today and learn how leasing your next multiphoton microscope can simplify equipment procurement.
All equipment brands/models are available
The Advantages of Excedr’s Multiphoton Microscope Leasing Program:
- Eliminates the upfront cost of purchasing equipment by spreading its cost over time
- Minimizes equipment downtime with included complete repair coverage and preventive maintenance
- Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
- Expedites the administrative work needed for instrument procurement and logistics
- Conserves working capital, enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
- Accommodates all manufacturer and model preferences
*Please consult your tax advisor to determine the full tax implications of leasing equipment.
Multiphoton microscopy is a fluorescence imaging technique, and is considered an optimal technique for the imaging of living, intact biological tissues.
MPM is also referred to as two-photon excitation microscopy, two-photon laser scanning microscopy, or nonlinear excitation microscopy and is regarded as an alternative to confocal microscopy.
Fluorescence microscopy is a technique that employs fluorescent tagging to illuminate specific parts of a biological sample rather than using an external light source. The fluorescent tags are then “activated” using electromagnetic radiation, illuminating thick specimens like organs and proteins. This allows image detection and magnification to occur, ultimately revealing structure and function.
However, while it is a powerful technique, fluorescent imaging has drawbacks. Background fluorescent light can negatively affect the image when imaging relatively thick samples. Suppose you only want to observe something on the surface of a thick specimen. In that case, the background fluorescent light from the thickness of the material often drowns out the fluorescent light from the material’s surface.
Confocal microscopy is notorious for causing photobleaching and photodamage throughout the specimen. It collects the fluorescence signal only from the focal plane, which causes the sample to absorb the remaining excitation light, creating significant problems in live samples.
Multiphoton microscopy solves this problem by using two photons as the excitation light, rather than just one photon, and by eliminating the contamination of the fluorescence signal by the excitation light. The photons used in MPM also have longer wavelengths, which have lower energy and penetrate further into the sample. This results in high-resolution, in vivo, 3D images of living cells and biological tissue, and reduces photobleaching and photo-toxicity.
MPM has applications in several fields, from biology, physiology, embryology, neurobiology, neuroscience, and cancer research to tissue engineering and dermatology.
Multiphoton Microscopy Variations, Techniques, & Price
Just like wide-field or confocal microscopy, MPM is based on fluorophore excitation. This excitation results in light emission, which can then be observed and analyzed.
Typically, a fluorophore is used to dye the sample, which becomes excited when it absorbs a single photon of a specific wavelength. However, in the case of MPM, two or three photons of longer wavelengths are focused at the sample area that’s been dyed through an objective lens, resulting in a specific area of the sample fluorescing.
Unlike traditional methods, which employ one photon, the photons have to strike the molecule within femtoseconds of each other. When they strike the molecule simultaneously, they excite that sample’s electrons to a higher, less stable state. This energy difference between the normal state and excited state is equal to the sum of the energies of the two photons used to excite it.
Due to the need for precision, the focused laser used in MPM must function at very high pulse rates. These pulsed lasers are often called femtosecond lasers. The absorption of two photons during the fluorophore’s excitation, along with the tightly focused laser, dramatically increases the probability of fluorescent emission.
This effectively restricts excitation fluorescence to a tiny focal point in the sample, resulting in increased rejection of out-of-focus light. The fluorescence produced is collected by a highly-sensitive detector, such as a photomultiplier tube.
This localized excitation and fluorescence are one of the reasons that multi-photon imaging is such a powerful tool. In addition, the light source used is typically infrared or near-infrared, which reduces scattering, further increasing MPM’s capabilities to generate high-resolution images.Another benefit of using two-photon microscopy is that it allows optical sectioning at greater depths. It can do this due to the extreme localization of the lasers used, which allows for deep tissue penetration.
Second-Harmonic Imaging (SHIM)
Maria Goeppet-Mayer: Two-Photon Absorption
Non-Linear & Two-Photon Excitation Microscope Leases to Fit Every Need
Regardless of the exact type of imaging system, funding a microscope acquisition can often be quite expensive. In fact, it might not be in your budget to purchase a multiphoton microscope outright.
Yet, not having the proper tissue imaging equipment can hamstring your laboratory’s ability to perform essential research. When budget is an issue, finding a workaround becomes critical to success.
Our lease program is one of those workarounds. You can access the technology you need and manage your cash more easily when you lease with us. On top of that, our lease program is brand agnostic and highly customizable, meaning you have the flexibility to choose whatever manufacturer you prefer.
Whether you need a multiphoton, wide-field, or confocal microscope, we can help. We offer a wide selection of leasing options and won’t slow your procurement process down with the typical requirements banks ask for when providing a loan.
Discuss your financing options in further detail by contacting us above. If you have an instrument quote from a manufacturer, request a lease estimate below, and we can begin creating custom lease terms for you and your laboratory.
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional monetary savings.
If you recently bought equipment, Excedr can offer you cash for your device and convert your purchase into a long-term rental. This is called a sale leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up money in your budget rather than tying it down to a fixed asset.
Two-Photon Excitation Microscope Manufacturers & Models
A1R MP+, A1 MP+
Ultima series, Ultima 2Pplus, Ultima In Vitro, Ultima Investigator
Movable Objective Microscope (MOM), 3P-MOM, DF-SCOPE