How Light Sheet Fluorescence Microscopy Works & How We Save You Time & Money
Excedr’s lease program can source all instrument types and accommodate any brand preferences your end-user might have despite the diversity in types. Request a light sheet fluorescence microscope lease estimate today and see how leasing your next light sheet microscope can simplify equipment procurement and budget management.
All equipment brands/models are available
The Advantages of Excedr’s Light Sheet Fluorescence Microscope (LSFM) Leasing Program:
- Eliminates the upfront cost of purchasing equipment by spreading its cost over time
- Minimizes equipment downtime with included complete repair coverage and preventive maintenance
- Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
- Expedites the administrative work needed for instrument procurement and logistics
- Conserves working capital, enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
- Accommodates all manufacturer and model preferences
*Please consult your tax advisor to determine the full tax implications of leasing equipment.
Light sheet microscopy is essential when imaging large, sensitive, living specimens that are easily damaged by light exposure.
As a fluorescence imaging technique, it utilizes a planar sheet of light in order to illuminate the live sample and excite fluorophores within a specific focal volume. The excitation light can either be created using a cylindrical lens or virtually generated using a scanning beam or dithering beam array.
The latter technique is normally referred to as digitally scanned laser light sheet microscopy (DSLM), while the former is called selective plane illumination microscopy, or SPIM.
Detection occurs along an axis perpendicular to that of illumination so that only the section being observed is lit, rather than the entire specimen or any out-of-focus features. This planar illumination technique produces optical sectioning, or clear images of multiple focal planes within the sample.
Furthermore, using the perpendicular axis reduces the amount of photo-damage, photo-bleaching, and phototoxicity to the specimen so that imaging can occur long-term.
It makes light sheet microscopy more ideal compared to processes like wide-field fluorescence microscopy that can damage your sample more rapidly and reduce long-term viability.
Optical sectioning offers a wide field of view, increased contrast, faster image acquisition speeds, and high axial resolution and spatial resolution. These capabilities support a variety of applications, including the real-time, three-dimensional imaging monitoring of neural responses in live organisms, and enable the 3D reconstruction of a 2D image by computationally combining the image data from a stack of images.
Light sheet microscopy’s principle benefits apply across multiple fields, including drug discovery, neurobiology, developmental biology, embryology, plant biology, and more.
Being able to 3D image everything from large, live organisms, tissue explants, to 3D cell cultures and single cells is crucial in many different areas of research.
This is especially true in developmental biology, where many model organisms are used to understand human development, such as fruit fly embryos (Drosophila melanogaster), an excellent model system for understanding the basic biology underlying our own embryonic development.
Another example of these organisms is the zebrafish, which is primarily used to study vascular development and disease.
Light sheet imaging has been pivotal in 3D examination, avoiding disruption or damage of the organism’s development cycle.
Methods, Configurations, & Light Sheet Microscope Price
It is first important to mention that there is no perfectly planar light sheet. The sheet is only approximated and given over a range of values using different methods such as static planar sheets or scanned beams that approximate a light sheet over time. Some examples of these methods include:
Bessel Beam/Airy Beam
Stripe Pattern Removal
Multiview Reconstruction & Deconvolution
Light Sheet Fluorescence Microscope Leases to Fit Every Need
Need to acquire a LSFM for your living specimen and cell imaging needs? The upfront costs of a light sheet fluorescence microscope may be higher than you are able or willing to pay. If that’s the case, you’re probably looking for an alternative to purchasing the microscope in order to create more flexibility within your budget.
Whatever your budgetary goals, when you lease a light sheet fluorescence microscope with Excedr, you can deploy the capital you do save on upfront costs to other areas of your business, such as hiring, buying consumables and reagents, or expanding your lab space.
Because our approval process is up to 12 weeks faster than traditional financing methods, you can acquire the equipment much quicker, accelerating your R&D or drug development and hitting milestones sooner.
If you have a quote from a manufacturer, we can put together a lease estimate for you. Or, if you’d just like to learn more about our leasing program, get in touch with us today.
Furthermore, we offer confocal microscopy leasing options in case you’re considering light sheet microscopy alternatives for imaging biological samples. If you’re unsure of whether a light sheet microscope or confocal microscope will suit your needs best, check out our confocal microscopy page.
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional monetary savings.
If you recently bought equipment, Excedr can offer you cash for your device and convert your purchase into a long-term rental. This is called a sale leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up money in your budget rather than tying it down to a fixed asset.
Light Sheet Fluorescence Microscope Manufacturers & Models
MuVi SPIM LS, MuVi SPIM CS, MuVI SPIM PM, InVi SPIM, InVi SPIM Lattice Pro, QuVi SPIM, LCS SPIM, TruLive3D Imager, Lux DATA
Lattice Lightsheet 7
UltraMicroscope Blaze, UltraMicroscope II,
LS1 Live system