How Confocal Microscopy Works & How We Save You Time & Money
Excedr can source all equipment types and can accommodate any model preferences your lab might have. Request a confocal microscope lease estimate today and see how leasing a confocal microscope can simplify equipment procurement and balance sheet management.
All equipment brands/models are available
The Advantages of Excedr’s Confocal Microscope Leasing Program:
- Eliminates the upfront cost of purchasing equipment by spreading its cost over time
- Minimizes equipment downtime with included complete repair coverage and preventive maintenance
- Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
- Expedites the administrative work needed for instrument procurement and logistics
- Conserves working capital, enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
- Accommodates all manufacturer and model preferences
*Please consult your tax advisor to determine the full tax implications of leasing equipment.
Confocal microscopy is an optical technique that uses fluorescence, an excitation light source, and pinhole spatial filters to obtain high-resolution images of biological and non-biological samples.
It is similar to wide-field fluorescence microscopy in that fluorescence optics are used. Unlike traditional fluorescence microscopes, however, confocal microscopy focuses a laser light source onto a defined spot at a specific depth within the sample. This creates the emission of fluorescent light at the exact point defined and, using a pinhole aperture inside the optical pathway, out-of-focus light is removed from the field of view. This leads to much clearer images being produced.
The term Confocal is derived from the conjunction of two focal planes: the objective lens focus point and the focus point where the aperture is placed.
Confocal imaging increases resolution and contrast through the use of the pinhole spatial filters. One pinhole is placed in a conjugate plane with a scanning point on the specimen, and a second pinhole is positioned in front of the detector (e.g., photomultiplier tube).
This effectively removes out-of-focus light and provides a crisper image with the maximal resolution possible for the objective lens being used.
Modern confocal microscopes offer high-power, high-resolution, and multicolor capabilities. They are commonly used to collect high-quality multispectral images of biological and non-biological samples across the visible range. Their maximum magnification level, in general, is 1000x when combining a 10x ocular with a 100x objective lens.
In addition, specialized confocal microscopes can image living cells, and work at very high speeds to perform live-cell imaging without causing severe photo damage, photobleaching, or phototoxicity. Confocal microscopes are even used to assist with in vivo preparations.
Furthermore, modern units are often designed as completely integrated confocal systems. In these systems, an optical microscope works together with a detector or multiple detectors, a computer, multiple laser systems, a wavelength selection device, and beam scanning assembly to produce images and obtain valuable information about a sample. These integrated systems are typically referred to as digital or video imaging systems.
Basic confocal microscopes and more robust systems are used extensively in the life sciences (e.g., biology and cell biology), materials science, and semiconductor inspection. When acquiring one, it’s essential to understand your needs, as this will inform whether or not you’ll want to lease a fully integrated system or something a little less complex.
Confocal Microscopy Techniques, Variations, & Costs
Confocal microscopes offers several advantages over conventional optical microscopes (e.g. light microscopes) including shallow depth of field, elimination of out-of-focus glare, and the ability to collect serial optical sections from thick specimens. Optical sectioning provides important structural information regarding both biological or non-biological samples.
Multiple subtypes of confocal microscopes have been developed.
Despite their differences, each subtype relies on fluorescence, or the generation of photons using controlled light sources and fluorophores, to produce an image. We’ll cover some of the subtypes below.
Laser Scanning (CLSM)
Spinning Disc (SDCLM)
Programmable Array Microscope (PAM)
Serotonin & Decision Making
We Offer Real-Time Confocal Laser Scanning Microscope Leases to Fit Every Need
Interested in leasing a laser scanning or spinning disk confocal microscope? We can help you procure it. Our microscope leases are designed to be flexible, customizable, and founder-friendly.
Additionally, we provide a wide range of other microscope solutions, including other light microscopy, X-ray, or Raman microscopy equipment. This includes confocal microscopy alternatives like two-photon and multiphoton microscopes and microscope systems.
Get in touch with us and we can talk about what specific equipment you would like to lease. Or, if you already know what you’d like, and have a quote from a manufacturer, request a lease estimate instead to get the application process started today.
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional monetary savings.
If you recently bought equipment, Excedr can offer you cash for your device and convert your purchase into a long-term rental. This is called a sale leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up money in your budget rather than tying it down to a fixed asset.