How Liquid Chromatographic Techniques Work & How We Save You Time & Money
Regardless of your liquid chromatography configuration requirements, the Excedr lease program can accommodate your lab’s equipment needs and model preferences. Request an estimate today and see how leasing can reduce your HPLC machine price.
All equipment brands/models are available
The Benefits of Excedr’s Liquid Chromatography Instrument Leasing Program:
- Eliminates the upfront cost of purchasing equipment by spreading its cost over time
- Minimizes equipment downtime with included complete repair coverage and preventive maintenance
- Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
- Expedites the administrative work needed for instrument procurement and logistics
- Conserves working capital, enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
- Accommodates all manufacturer and model preferences
*Please consult your tax advisor to determine the full tax implications of leasing equipment.
Liquid chromatography (LC) is essential in many laboratories for the separation, identification, purification, and quantification of various compounds in different matrices.
Traditional liquid chromatography (LC) relied on gravity to get the mobile phase and sample through the column. But as smaller particles were used to improve separation power and higher pressures were needed to create the desired flow through the column, high-pressure liquid chromatography, or HPLC, was developed. Eventually, as advances in instrumentation continued and performance improved, the acronym HPLC came to stand for high-performance liquid chromatography.
Systems that operate at pressures up to 35,000 psi are known as medium-pressure chromatography systems, and are typically used as part of a larger laboratory workflow to separate, prepare, or quantitate samples. Pumps vary in pressure capacity, but their performance is measured on their ability to yield a consistent and reproducible flow rate. Pressure may reach as high as 60 MPa (6000 lbf/in2), or about 600 atmospheres.
Modern HPLC systems have been improved to work at much higher pressures, and therefore are able to use much smaller particle sizes in the columns (<2 μm). These “Ultra-High-Performance Liquid Chromatography” systems or UHPLCs can work at up to 120 MPa (17,405 lbf/in2), or about 1200 atmospheres. The term “UPLC” is a trademark of the Waters Corporation, but is sometimes used to refer to the more general technique of UHPLC.
In addition to UHPLC, there is another subset of liquid chromatography called fast protein liquid chromatography (FPLC). FPLC is generally applied only to proteins, however, because of the wide choice of resins and buffers, it has broad applications. In contrast to HPLC, the buffer pressure used is relatively low, typically less than 5 bar, but the flow rate is relatively high, typically 1-5 ml/min. FPLC can be readily scaled from analysis of milligrams of mixtures in columns with a total volume of 5ml or less to industrial production of kilograms of purified protein in columns with volumes of many liters.
Not to be mistaken with FPLC, there are also flash chromatography systems on the market which minimize human involvement in the purification process. These systems are typically referred to as LPLC, low-pressure liquid chromatography, and were designed to address the extremely time-consuming column chromatography stage which oftentimes bottlenecks the productivity of process labs.
Lastly, there’s ion chromatography. Ion chromatography (IC) systems separate charged particles from a liquid and measure their concentration. IC systems can analyze particles such as anions, cations, organic salts, and proteins; IC is also very closely related to HPLC. They are typically used in environmental, manufacturing, food, pharmaceutical, and chemical industries.
Components & Types of Liquid Chromatography Systems
Every HPLC instrument has the following key components:
- Sample injector
- Data handling computer
For applications that require close control of column temperature, column thermostats are usually included in the LC system. The other advantage of using a thermostat is that more reproducible chromatograms are usually obtained by maintaining column temperature. While organic-based chromatography usually involves column heating, the opposite is true for separating proteins.
There are many detectors for LC, some of which include:
- photodiode array (PDA)
- mass spectrometer (LC-MS machine)
- refractive index
- electrochemical, and
- evaporative light scattering (ELSD).
As for HPLC system prices, simple setups with a basic detector, injector, and pump are relatively affordable. This is especially the case if you opt for HPLC over UHPLC. However, if you increase the number of detection modules, add an autosampler, and upgrade to a high-pressure pump, the cost rises dramatically.
Whether you’re looking to combine a liquid chromatography system with IR spectrometry or lease a nitrogen evaporator to prepare samples, we can help. Fill out our contact form or give us a call at (510) 982-6552 and we can discuss your specific equipment needs.
Lease Any Chromatography Instrument That Suits Your Needs
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional cash savings.
If you recently purchased equipment, Excedr can offer you cash for your instrument and convert your purchase into a long-term rental. This is called a sale leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up cash in your budget rather than tying it down to the instrument.
Liquid Chromatography System Manufacturers & Models on the Market
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1220 Infinity II, 1260 Infinity II, 1260 Infinity II Prime, 1290 Infinity II
NGC 10 Medium-Pressure, NGC 100 Medium-Pressure, NGC Quest 10, NGC Quest 10 Plus, NGC Quest 100, NGC Quest 100 Plus, NGC Scout 10, NGC Scout 10 Plus, NGC Scout 100, NGC Scout 100 Plus, NGC Discover 10, NGC Discover 10 Pro, NGC Discover 100, NGC Discover 100 Pro, BioLogic™ LP Low-Pressure
Alliance HPLC, Arc HPLC, Breeze QS HPLC, ACQUITY UPLC/HPLC, Arc Premier, ACQUITY Arc Systems, ACQUITY UPC2, Supercritical Fluid Chromatography, ACQUITY Advanced Polymer, ACQUITY UPLC M-Class, Patrol UPLC Process Analysis
ExionLC AC, ExionLC AD, M3 MicroLC LC-MS, NanoLC 400, cHiPLC, CESI 8000 Plus
LC 300 UHPLC, LC 300 HPLC, NexSAR Speciation Analysis Ready HPLC, QSight Triple Quad, QSight Triple Quad 400 Series
Nexera Series, Nexera 40 Series, i-Series, i-Series Integrated UHPLC, Nexera Prep HPLC
CombiFlash EZ Prep, CombiFlash Rf+, CombiFlash Rf+ Purlon, CombiFlash Rf+ Lumen, CombiFlash Rf 150, CombiFlash Torrent, ACCQPrep HP150
Vanquish Horizon, Vanquish Flex, Vanquish Core, Vanquish Duo, Vanquish Online SPE, Vanquish Loop, Vanquish Trap Heart-Cut, Vanquish Simple Switch, Vanquish Method Development, Vanquish Neo, UltiMate 3000 RS, UltiMate 3000 SD, UltiMate 3000 Basic, UltiMate 3000 Semi-Preparative, UltiMate 3000 RSLCnano, Transcend LX, Transcend TLX
VERITY Oligonucleotide Purification System, VERITY Compact LC System, VERITY 281 HPLC System, VERITY 271 HPLC System, VERITY 241 HPLC System
AKTA pure, AKTA start, AKTA go, AKTA avant, AKTA pilot, AKTA process