How Liquid Chromatographic Techniques Work & How We Save You Time & Money
Regardless of your liquid chromatography configuration requirements, the Excedr lease program can accommodate your lab’s equipment needs and model preferences. Request an estimate today and see how leasing can reduce your HPLC machine price.
All equipment brands/models are available
Here is how our LC instrument leasing program benefits you:
- Stretching out payments over time removes the burdensome upfront cost of buying equipment
- Payments may be 100% tax deductible*, which makes leasing even more affordable
- It is cheaper to lease through our program than to purchase outright
- Our worry-free repair coverage minimizes instrument downtime
- We take care of the admin work associated with equipment sourcing and repairs
- With the cash saved through our program, companies are better able to reinvest in their core business and operations (staffing, inventory, marketing/sales, etc.)
*Please consult your tax advisor to determine the full tax implications of leasing equipment.
Liquid chromatography (LC) is essential in many laboratories for the separation, identification, purification, and quantification of various compounds in different matrices.
Components & Types of Liquid Chromatography Systems
“Classical” LC relied on gravity to get the mobile phase and sample through the column. But as smaller particles were used to improve separation power and higher pressures were needed to create the desired flow through the column, high-pressure liquid chromatography, or HPLC, was developed. Eventually, as advances in instrumentation continued and performance improved, the acronym HPLC came to stand for high-performance liquid chromatography. Systems that operate at pressures up to 35,000 psi are known as medium-pressure chromatography systems, and are typically used as part of a larger laboratory workflow to separate, prepare, or quantitate samples. Pumps vary in pressure capacity, but their performance is measured on their ability to yield a consistent and reproducible flow rate. Pressure may reach as high as 60 MPa (6000 lbf/in2), or about 600 atmospheres.
Modern HPLC systems have been improved to work at much higher pressures, and therefore are able to use much smaller particle sizes in the columns (<2 μm). These “Ultra-High-Performance Liquid Chromatography” systems or UHPLCs can work at up to 120 MPa (17,405 lbf/in2), or about 1200 atmospheres. The term “UPLC” is a trademark of the Waters Corporation, but is sometimes used to refer to the more general technique of UHPLC. In addition to UHPLC, there is another subset of liquid chromatography called fast protein liquid chromatography (FPLC). FPLC is generally applied only to proteins, however, because of the wide choice of resins and buffers, it has broad applications. In contrast to HPLC, the buffer pressure used is relatively low, typically less than 5 bar, but the flow rate is relatively high, typically 1-5 ml/min. FPLC can be readily scaled from analysis of milligrams of mixtures in columns with a total volume of 5ml or less to industrial production of kilograms of purified protein in columns with volumes of many liters.
Not to be mistaken with FPLC, there are also flash chromatography systems on the market which minimize human involvement in the purification process. These systems are typically referred to as LPLC, low-pressure liquid chromatography, and were designed to address the extremely time-consuming column chromatography stage which oftentimes bottlenecks the productivity of process labs. Lastly, there’s ion chromatography. Ion chromatography (IC) systems separate charged particles from a liquid and measure their concentration. IC systems can analyze particles such as anions, cations, organic salts, and proteins; IC is also very closely related to HPLC. They are typically used in environmental, manufacturing, food, pharmaceutical, and chemical industries.
With all of that being said, every HPLC instrument has the following key components:
- Sample injector
- Data handling computer
For applications that require close control of column temperature, column thermostats are usually included in the LC system. The other advantage of using a thermostat is that more reproducible chromatograms are usually obtained by maintaining column temperature. While organic-based chromatography usually involves column heating, the opposite is true for separating proteins.
There are many detectors for LC, some of which include:
- photodiode array (PDA)
- mass spectrometer (LC-MS machine)
- refractive index
- electrochemical, and
- evaporative light scattering (ELSD).
As for HPLC system prices, simple setups with a basic detector, injector, and pump are relatively affordable. This is especially the case if you opt for HPLC over UHPLC. However, if you increase the number of detection modules, add an autosampler, and upgrade to a high-pressure pump, the cost rises dramatically.
Whether you’re looking to combine a liquid chromatography system with IR spectrometry or lease a nitrogen evaporator to prepare samples, we can help. Fill out our contact form or give us a call at (510) 982-6552 and we can discuss your specific equipment needs.
Lease Any Chromatography Instrument That Suits Your Needs
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional cash savings.
If you recently purchased equipment, Excedr can offer you cash for your instrument and convert your purchase into a long-term rental. This is called a sale leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up cash in your budget rather than tying it down to the instrument.
Liquid Chromatography System Manufacturers & Models on the Market
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1220 Infinity II, 1260 Infinity II, 1260 Infinity II Prime, 1290 Infinity II
NGC 10, NGC 100, BioLogic LP System, Profinia Affinity, NGC Discover
ACQUITY UPLC, ACQUITY UPLC H-Class, ACQUITY UPLC I-CLASS, ACQUITY UPLC M-CLASS, ACQUITY UPC2, ACQUITY Arc UHPLC, Alliance HPLC, ACQUITY Arc Systems, ACQUITY Arc Bio System, ACQUITY Arc Multi-Dimensional
ExionLC AC, ExionLC AD, M3 MicroLC LC-MS, NanoLC 400, cHiPLC, CESI 8000 Plus
Flexar UHPLC, Flexar LC, NexSAR HPLC
Nexera X3, Nexera X2, Nexera MX Multiplex, Nexera XR, Prominence, i-Series, Prominence-i, Nexera TQ, Nexera SQ, Nexera SR, Nexera Quaternary, Nexera MP, Nexera Method Scouting, Prominence nano
CombiFlash EZ Prep, CombiFlash Rf+, CombiFlash Rf+ Purlon, CombiFlash Rf+ Lumen, CombiFlash Rf 150, CombiFlash Torrent, ACCQPrep HP150
Vanquish Horizon, Vanquish Flex Binary, Vanquish Flex Quaternary, UltiMate 3000 RS, UltiMate 3000 SD, UltiMate 3000 RSLCnano, UltiMate 3000 Basic, UltiMate 3000 Semi-Preparative
VERITY Oligonucleotide Purification System, VERITY Compact LC System, VERITY 281 HPLC System, VERITY 271 HPLC System, VERITY 241 HPLC System
AKTA pure, AKTA start, AKTA go, AKTA avant, AKTA pilot, AKTA process