How Liquid Chromatography Works & How Excedr Saves You Time & Money
Regardless which brand or model you’re interested in, Our lease program can accommodate your lab’s equipment needs and preferences. Request an estimate today and see how leasing an LC system can reduce upfront costs and simplify cash flow management.
All equipment brands/models are available
The Benefits of Excedr’s Liquid Chromatography System Leasing Program:
- Eliminates the upfront cost of purchasing equipment by spreading its cost over time
- Minimizes equipment downtime with included complete repair coverage and preventive maintenance
- Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
- Expedites the administrative work needed for instrument procurement and logistics
- Conserves working capital, enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
- Accommodates all manufacturer and model preferences
*Please consult your tax advisor to determine the full tax implications of leasing equipment.
Liquid chromatography is a form of column chromatography used in countless analytical laboratories to separate, identify, purify, and quantify various biomolecules (nucleic acids, proteins, etc.) and small molecules.
It is considered an essential part of many analytical workflows.
Since its development, this technique has gone through several evolutions. Initially, LC relied on gravity to get the mobile phase, a solvent that picks up the sample and moves it through the column or stationary phase. However, as smaller particles began to be used more often, higher pressures were needed to create the desired flow through the column to improve separation power. To create the necessary flow rate, high-performance liquid chromatography (HPLC), formerly called high-pressure liquid chromatography, was developed.
In practice, this powerful separation technique is performed using a chromatograph comprising several essential components. A pump, in conjunction with a mobile phase (solvent), is used to move a sample mixture (analyte) at high pressure through a column with chromatographic packing material (resin) known as the stationary phase.
As the sample moves through the column, the sample’s compounds interact with the two phases at different strengths and are separated based on their varying affinity, or polarity, for the mobile or stationary phase. The molecules within the sample that have the least interaction with the stationary phase or, inversely, the most interaction with the mobile phase will exit the column faster.
Furthermore, sample retention time (the interval between the injection of a sample and the detection of substances in that sample) will vary depending on the interaction between the stationary phase, the molecules being analyzed, and the solvent or solvents used.
The molecules are collected in a series of test tubes, either manually or with a fraction collector upon exit. Next, an analysis of each fraction is performed to identify the compound present. Colored compounds can be detected by visual observation; however, colorless compounds are detected using various types of specialized liquid chromatography detectors.
HPLC can separate and identify compounds that are present in any sample that can be dissolved in a liquid in trace concentrations as low as parts per trillion (ppt). Because of this versatility, HPLC is used in various industrial and scientific applications, such as pharmaceutical, environmental, forensics, and chemicals.
Modes, Liquid Chromatography System Components, & Prices
There are several types of liquid chromatography modes used in laboratories today to perform various analyses. Depending on the stationary and mobile phase choice, some standard modes are used to separate most compounds: reversed-phase, normal phase, ion exchange, and size exclusion.
The basic instrumentation of each system is generally the same, regardless of the separation mode being used. Instrumentation includes a pump, sample injector, chromatography column, detector, and data handling computer and software.Below, we will cover some LC modes used and review the basic components of an LC system.
Chromatography Data System
Lease Any Brand of HPLC System That Suits Your Needs
Our lease program can accommodate if you’re interested in acquiring a chromatography instrument or system for your laboratory, whether it be liquid chromatography, gas chromatography, or thin-layer chromatography.
Lastly, if you need any preparative instruments, such as a nitrogen evaporator or nucleic acid purification instrument, we can help with that as well. Fill out our contact form or give us a call at (510) 982-6552, and we can discuss your specific equipment needs.
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional cash savings.
If you recently purchased equipment, Excedr can offer you cash for your instrument and convert your purchase into a long-term rental. This is called a sale leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up cash in your budget rather than tying it down to the instrument.