How Ion Exchange Chromatography Works & How We Save You Time & Money
Despite the diversity in methods, the Excedr lease program is able to source all instrument types and can accommodate any brand preferences your end-user might have. Request an estimate today and see how leasing is an excellent alternative to buying an ion chromatography system.
All equipment brands/models are available
The Benefits of Excedr’s Ion Chromatograph Instrument Leasing Program:
- Eliminates the upfront cost of purchasing equipment by spreading its cost over time
- Minimizes equipment downtime with included complete repair coverage and preventive maintenance
- Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
- Expedites the administrative work needed for instrument procurement and logistics
- Conserves working capital, enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
- Accommodates all manufacturer and model preferences
*Please consult your tax advisor to determine the full tax implications of leasing equipment.
Ion chromatography (IC), also commonly referred to as ion exchange chromatography (IEX), is an analytical process for separating ions and polar molecules using ion exchange in a material.
It is a modified version of high-performance liquid chromatography (HPLC) and is based on the phenomenon of attraction between opposite charges. In other words, it’s based on the theory that opposites attract!
The method combines both chromatographic and ion equilibrium theories into one technique in order to separate ionic species. It is commonly used in biomolecule purification, and there are several specialized modes used in laboratories today. Scientists and researchers rely on ion chromatography for its usefulness at all stages and levels of purification, it’s concentrating and controllable capabilities, as well as its high selectivity, capacity, and recovery.
Furthermore, it is able to look at major anions such as fluoride, chloride, nitrate, nitrite, and sulfates, as well as major cations such as lithium, sodium, ammonium, potassium, calcium, and magnesium. However, this chromatography method must be performed in an environment that is one unit away from the isoelectric point (pl) of a protein.
The principle of ion exchange chromatography is as follows:
“A crude sample containing charged molecules is used as the liquid phase. When it passes through the chromatographic column, molecules bind to oppositely charged sites in the stationary phase.The molecules separated on the basis of their charge are eluted using a solution of varying ionic strength. By passing such a solution through the column, highly selective separation of molecules according to their different charges takes place.”
The stationary phase is typically a polymeric resin. It’s selected so that it has a particular charge and attracts the sample molecules with the opposite charge. This phase is also termed as the ion exchanger. The ion exchanger can be two types, either a cation exchanger or an anion exchanger. Cation exchangers have a negative charge and attract positively charged cations, while anion exchangers are positively charged and attract negatively charged anions.
The mobile phase, or eluent, is a typically a solution—an inorganic salt dissolved in a suitable solvent—which introduces the ions that need to be separated into the system. Aqueous acid and base solutions, as well as organic, non-aqueous solutions can also be used as the mobile phase. The solution acts as the carrier portion of the mobile phase, and carries the analytes through the ion chromatograph.
There are a number of ion chromatography analyses performed in laboratories today, some of which include ammonia, cyanide, transition metals, and pharmaceuticals analysis. Yet, despite each method’s distinct uses, ion chromatography instrumentation is composed of several standard components and is similar to that of HPLC: a pumping system, chromatography columns, and a detecting system.
Ion Exchange Chromatography Methods & Components
Suppressed & Non-Suppressed
NASA’s Ion Chromatography Chip
Ion Exchange Chromatography Leases to Fit Every Need
At Excedr, we can help you acquire new pieces of equipment at a fraction of the upfront cost. Furthermore, our lease program provides flexibility and customizability, ensuring you pay manageable, affordable rates for the duration of the lease term.
From ion chromatography systems to mass spectrometry instruments to protein purification devices, we’ve got you covered. Connect with us by calling (510) 982-6552 or filling out our contact form and we can discuss your options in depth.
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional monetary savings.
If you recently bought equipment, Excedr can offer you cash for your device and convert your purchase into a long-term rental. This is called a sale leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up money in your budget rather than tying it down to a fixed asset.