How FPLC Systems Work & How Leasing One Can Benefit Your Lab

Excedr Chromatography FPLC System

Excedr’s lease program can source virtually all instrument types and accommodate any brand preferences you might have. Request an FPLC system lease estimate today and see how leasing can benefit your laboratory.

All equipment brands/models are available

The Advantages of Excedr’s FPLC Leasing Program:

  • Eliminates the upfront cost of purchasing equipment by spreading its cost over time
  • Minimizes equipment downtime with included complete repair coverage and preventive maintenance
  • Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
  • Expedites the administrative work needed for instrument procurement and logistics
  • Conserves working capital,  enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
  • Accommodates all manufacturer and model preferences

*Please consult your tax advisor to determine the full tax implications of leasing equipment.

chromatography system diagram

Fast protein liquid chromatography (FPLC) is a medium- or low-pressure liquid chromatography method for purifying, and occasionally analyzing, different mixtures of large biomolecules.

Biomolecule mixtures can include proteins, peptides, oligonucleotides, DNA, and RNA. Because biomolecules are highly sensitive and can’t withstand the type of high pressure, temperature, or solvents used in high-performance liquid chromatography (HPLC), FPLC is employed. FPLC is also used to collect separated compounds of interest and identify protein profiles within single proteins. 

You may hear FPLC referred to as medium-pressure chromatography, biochromatography, or biopurification, but the title doesn’t change the process. It’s an important method for many researchers in the life sciences and biotech. 

Separating a sample within a mixture depends on each molecule’s specific interaction with the stationary and mobile phases used during chromatography. FPLC utilizes a wide range of resins, composed of many small coated beads, for its stationary phase, and a an aqueous solution or buffer for its mobile phase. 

The targeted protein adheres to the resin as it flows through the separation column and the buffer carries any other molecules that are present out of the system. It’s typical to see a mixture of buffers used to accomplish this elution. 

For example, having flushed the system, a secondary buffer, called an eluent, will separate the protein of interest from the resin and pull it into a detection system for measurement, recording the concentration levels of the target protein in the sample.

FPLC is a type of preparative technique, unlike HPLC which provides quantitative and qualitative analysis of liquid samples. It is used in all sorts of settings, from academia to research facilities to industrial manufacturing. 

It’s versatility is due in part to its simplicity, rapidity, and reliability as a technique, producing consistent results that end-users can count on. 

Furthermore, its ability to support multiple columns simultaneously is based on its use of low pressure and offers a wide flow range when combined with multiple pumps, making the entire process efficient and highly scalable. 

Some of the most common FPLC systems on the market include the AKTA FPLC system, offered by Cytiva—formerly GE Healthcare and Amersham Biosciences—and the AZURA® FPLC system, offered by KNAUER. (And guess what? We lease them.)

FPLC System Components, Modes, & Price

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Fast protein liquid chromatography chromatographs operate using several key components and chromatographic modes.

Components
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FPLC systems consist of the following features:

  • Program controller: This controls many aspects of the system, from separation parameters to data collection.
  • Pump: It is typical to see two pumps used to control the amount of each buffer introduced during liquid chromatography. This is often a type of piston pump. Occasionally, a single pump will be used where the buffers are drawn from their separate reservoirs into a proportioning valve attached to a mixing chamber.
  • Injection valve: This valve connects the sample loop and mixer to the column. The valve is able to load the sample into the injection loop, which injects the sample into the column.
  • Injection loop: This tubing is filled with the sample solution before it is injected into the column.
  • Column: Made of glass or plastic, this chromatographic column is longer than one typically used during HPLC, but has a large internal diameter, as the samples used in FPLC are larger. The column is packed with the resin and buffer solution, with a small opening on one end to retain the beads while letting the dissolved proteins and buffer pass through.
  • Flow cells: The eluent passes through one or more flow cells which measure the concentration of protein in the eluent using UV light, salt concentration using conductivity, and even measure the level of pH. These monitors allow for detection of proteins when individually present in the eluent.
  • Fraction Collector: This component is made up of small containers such as test tubes that collect the eluent in fixed volumes or specific fractions based on peak protein concentration.
  • Monitor: The monitor, or recorder, consists of a computer and interface with a display. It identifies and records when concentrations of biomolecules are present.
 

Ion Exchange (IEX)
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Ion exchange chromatography is used to separate molecules based on their specific charge, which depends on the pH or buffer.

If a protein is more charged, it will be more likely to bind with an oppositely charged resin. Depending on salt concentration, a protein will take shorter or longer to elute from the resin as the salt ions compete with the molecule for adherence to the resin.

 

Affinity (AC)
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In affinity chromatography—a separation method used in FPLC—using a substrate that is linked with the stationary phase allows a sample to pass through and bind to the solid substrate according to its affinity for the substrate used.

The compounds that haven’t bound are then washed out using an aqueous solution. The remaining compounds that are bound to the substrate are then eluted using a solvent with a certain pH or salt concentration.

 

Size Exclusion (SEC)
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Size exclusion chromatography is also referred to as gel filtration chromatography, and consists of a gel medium of specifically sized beads that separate the sample mixture as it passes through the FPLC column.

Molecules that are larger cannot pass through and are eluted from the column, while the smaller molecules diffuse into the gel medium and remain fixed in place. This results in separation based on size and molecular weight, as elution happens in order of linear weight.

 

We Offer Biochromatography Leases to Fit Every Need

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Purifying proteins has played an active role in many scientific fields, specifically biotechnology. Without chromatography, scientists wouldn’t be able to so easily separate, identify, and purify a mixture’s components for qualitative and quantitative analysis. 

Any laboratory that deals with protein purification and analysis requires using a chromatograph at some point or other, which is why having the ability to perform methods such as FPLC will always be critical to research.

If acquiring an FPLC chromatography system for your company is proving financially challenging, lease with Excedr. We offer comprehensive lease programs that bypass the costly upfront charges required when buying a piece of equipment outright. Furthermore, if you’re in need of a high-pressure system, we lease HPLC systems as well.

Request a lease estimate today and let us know how we can help you acquire liquid chromatography solutions for your laboratory.

Operating Lease

This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional monetary savings.

Sale-Leaseback

If you recently bought equipment, Excedr can offer you cash for your device and convert your purchase into a long-term rental. This is called a sale-leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up money in your budget rather than tying it down to a fixed asset.


FPLC System Manufacturers & Models on the Market
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Cytiva:
ÄKTA start, ÄKTA go, ÄKTA pure, ÄKTA avant

Bio-Rad Laboratories:
NGC 10 Medium-Pressure Chromatography Systems, NGC 100 Medium-Pressure Chromatography Systems, BioLogic DuoFlow, BioLogic Low-Pressure Liquid Chromatography Systems

KNAUER:
AZURA Bio Lab System, AZURA Bio Lab System Advanced, AZURA Bio compact AC system, AZURA Bio compact SEC system, AZURA Pilot system

 


Operating Capital Benefits

Operating Capital Benefits

Excedr's operating lease structure allows you to keep your business credit line open for expansions, staffing, and other operational expenses. Additionally, it strengthens the cash flow of your business and keeps cash reserves free for business development opportunities.

Effects on Credit

Effects on Credit

Leasing / renting provides you with non-dilutive financing and does not hinder your future borrowing ability. You're able to acquire the equipment you need without the baggage associated with traditional financing.

Used Equipment

Used Equipment

Unlike traditional financing and leasing companies, the Excedr program can accommodate refurbished/reconditioned equipment in addition to demo units. If you are looking for additional cost-savings, we recommend considering this option.

Speed of Approval

Speed of Approval

Excedr's program allows you to respond quickly as your need for equipment and technology arises. You can be approved with minimal documentation and have the equipment you need in operation and generating revenue for your business quickly.