How FPLC Systems Work & How Leasing One Can Benefit Your Lab
Excedr’s lease program is able to source all instrument types and can accommodate any brand preferences you or your end-user might have. Request a lease estimate today and see how leasing can provide you access to the latest in FPLC chromatography technology.
All equipment brands/models are available
The Advantages of Excedr’s FPLC Leasing Program:
- Eliminates the upfront cost of purchasing equipment by spreading its cost over time
- Minimizes equipment downtime with included complete repair coverage and preventive maintenance
- Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
- Expedites the administrative work needed for instrument procurement and logistics
- Conserves working capital, enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
- Accommodates all manufacturer and model preferences
*Please consult your tax advisor to determine the full tax implications of leasing equipment.
Fast protein liquid chromatography (FPLC) is a medium- or low-pressure liquid chromatography method for purifying, and occasionally analyzing, different mixtures of large biomolecules.
It is also used to collect separated compounds of interest and identify protein profiles within single Biomolecule mixtures can include proteins, peptides, oligonucleotides, DNA, and RNA. Because biomolecules are highly sensitive and can’t withstand the type of high pressure, temperature, or solvents used in high-performance liquid chromatography (HPLC), FPLC is employed. FPLC is also used to collect separated compounds of interest and identify protein profiles within single proteins.
You may hear FPLC referred to as medium-pressure chromatography, biochromatography, or biopurification, but the title doesn’t change the process. It’s an important method for many researchers in the life sciences and biotech.
Separating a sample within a mixture depends on each molecule’s specific interaction with the stationary and mobile phases used during chromatography. FPLC utilizes a wide range of resins, composed of many small coated beads, for its stationary phase, and a an aqueous solution or buffer for its mobile phase.
The targeted protein adheres to the resin as it flows through the separation column and the buffer carries any other molecules that are present out of the system. It’s typical to see a mixture of buffers used to accomplish this elution.
For example, having flushed the system, a secondary buffer, called an eluent, will separate the protein of interest from the resin and pull it into a detection system for measurement, recording the concentration levels of the target protein in the sample.
FPLC is a type of preparative technique, unlike HPLC which provides quantitative and qualitative analysis of liquid samples. It is used in all sorts of settings, from academia to research facilities to industrial manufacturing.
Its versatility is due in part to its simplicity, rapidity, and reliability as a technique, producing consistent results that end-users can count on.
Furthermore, its ability to support multiple columns simultaneously is based on its use of low pressure and offers a wide flow range when combined with multiple pumps, making the entire process efficient and highly scalable.
Some of the most common FPLC systems on the market include the AKTA FPLC system, offered by Cytiva—formerly GE Healthcare and Amersham Biosciences—and the AZURA® FPLC system, offered by KNAUER. (And guess what? We lease them.)
FPLC System Components, Modes, & Price
Fast protein liquid chromatography chromatographs operate using several key components and chromatographic modes.
We Offer Biochromatography Leases to Fit Every Need
Purifying proteins has played an active role in many scientific fields, specifically biotechnology. Without chromatography, scientists wouldn’t be able to so easily separate, identify, and purify a mixture’s components for qualitative and quantitative analysis.
Any laboratory that deals with protein purification and analysis requires using a chromatograph at some point or other, which is why having the ability to perform methods such as FPLC will always be critical to research.
If acquiring an FPLC chromatography system for your company is proving financially challenging, lease with Excedr. We offer comprehensive lease programs that bypass the costly upfront charges required when buying a piece of equipment outright. Furthermore, if you’re in need of a high-pressure system, we lease HPLC systems as well.
Request a lease estimate today and let us know how we can help you acquire liquid chromatography solutions for your laboratory.
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional monetary savings.
If you recently bought equipment, Excedr can offer you cash for your device and convert your purchase into a long-term rental. This is called a sale-leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up money in your budget rather than tying it down to a fixed asset.