How Western Blot & Gel Imaging Systems Work & How We Save You Time & Money

Excedr - Western Blot Imaging Systems

Despite the diversity in types, the Excedr lease program is able to source all instrument types and can accommodate any brand preferences your end-user might have. Request an estimate today and see how leasing can positively affect your western blot imaging systems’ price.

All equipment brands/models are available

The Advantages of Excedr’s Western Blot Imager Leasing Program:

  • Eliminates the upfront cost of purchasing equipment by spreading its cost over time
  • Minimizes equipment downtime with included complete repair coverage and preventive maintenance
  • Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
  • Expedites the administrative work needed for instrument procurement and logistics
  • Conserves working capital,  enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
  • Accommodates all manufacturer and model preferences

*Please consult your tax advisor to determine the full tax implications of leasing equipment.

a cell, a microplate well, antibodies, and a dot with 6 lines through it

Western blot imaging is a type of assay commonly used in the lab to perform research or offer services related to molecular biology.

Specifically, western blot imaging systems allow for the visualization and study of target proteins within a mixture of proteins. 

Gel imaging is also commonly used in molecular biology, as it enables researchers to study nucleic acids using the same gel-based techniques used in western blotting immunoassays. Many systems available today integrate gel imaging and western blot documentation in one instrument.

Each technique and instrument type offers imaging and documentation capabilities regarding specific nucleic acid and proteins that are suspended within agarose or polyacrylamide gels. These gel samples are generally stained using ethidium bromide or similar fluorophores.

Traditional detection methods include using X-ray film or a transilluminator to detect protein or nucleic acid levels. For example, in chemiluminescent western blots, X-ray film is used to detect target proteins and requires the use of a darkroom. 

However, more modernized systems and methods rely on a digital charge-coupled device (CCD) imager to detect. Both modes of detection are commonly used, as each offers valuable benefits to the end-user.

When it comes to digital imaging, western blot and gel doc systems generally include advanced software which simplifies the research process by automatically acquiring the data, analyzing it, and validating it. This is extremely useful when dealing with the many nucleic acid and protein staining modes, including fluorescent, colorimetric, radioisotopic, and chemiluminescent. 

By digitizing the gels, you ensure the benefits of accurate sample quantification and simple, practical data storage. This process is even more straightforward as most of these systems are compatible with PC and Mac operating systems.

Types, Benefits, & Gel Doc System Cost

an outstretched hand face up holding the end of a DNA double helix

Western blot and gel imaging systems can simplify complicated research in the life sciences, allowing laboratories to leverage the images for a presentation or store them more conveniently. There are several types of systems used today. The general steps of western blotting are as follows:

First, the researcher must prepare the protein sample by mixing it with a detergent called sodium dodecyl sulfate, making the proteins unfold into linear chains and coating them with a negative charge.

Next, the protein molecules are separated according to their sizes using a method called gel electrophoresis. After separation has been completed, the proteins are transferred from the gel onto a blotting membrane, which holds all of the protein bands originally on the gel.

Afterwards, the membrane goes through a treatment called blocking. Blocking prevents any nonspecific reactions from occurring. The membrane is then incubated with the primary antibody, which specifically binds to the protein of interest.

Following incubation, any unbound primary antibody is washed away, and the membrane is incubated again; however, this time, it is incubated with a secondary antibody. This secondary antibody is used to recognize and bind to the primary antibody. This is because the secondary antibody is linked to a reporter enzyme that produces color or light, which allows it to be easily detected and imaged. 

By following these steps, researchers can cause a specific protein to be detected from among a mixture of proteins.

The various types of systems and films used in western blot and gel imaging are described below.

Gel Doc Systems

While there are many choices for gel documentation systems, the one you choose should be tailored to fit your current and future needs. The range of features available makes choosing your customization options an integral part of the leasing process. There are several main features you should know about:

  • Data Analysis & Output: There are many modes and options regarding analysis and output, and by tailoring them to your needs, you can improve the ease with which data evaluation, sharing of data, custom reporting, and image generation are completed.
  • Multi Fluorescent Western Blot: This feature allows for simultaneously analyzing multiple proteins in a single blot. Doing so saves time, as it eliminates the need for loading the sample into multiple wells or reprobing or stripping the blot. This also increases accuracy as it removes human error when reloading the blot.
  • Signal Accumulation Mode: This refers to the accumulation of chemiluminescence data over a period of time, allowing the measurements to be taken as it develops. This ability to capture and analyze the time course for the signals makes it different from film-based systems, which only offer an image at a point in time.
 

Film Autoradiography

Autoradiography of Western blots requires a specific type of X-ray film for the radioisotope samples involved. These samples are most common in medicine, pharmacology, and research-based laboratories, so its typical to see film western blot imaging in these fields.

The target proteins are exposed to the autoradiography film and transferred to the gel membrane for detection. However, these days, it is more common to use nonradioactive detection methods whenever possible, especially chemiluminescence, which can also be done using x-ray film.

 

Multiplex

Multiplex assays use fluorescence to illuminate the blotting membrane, detect signals, capture images, and analyze. This makes multiplex fluorescent western blotting extremely useful in experiments that focus on imaging and detecting multiple fluorescent signals or on detecting colorimetric, luminescent, and radioisotopic signals.

 

Chemiluminescence

This type, primarily used in western blotting, focuses on separating proteins based on their molecular weights. This specific application is popular because it is highly sensitive and often used with techniques that offer improved speed, signal stability, and ease of use.

 

Laser

This is a variation of the multiplex type offering high sensitivity and precise quantitation for radioisotopes, as well as a variety of options regarding emission filters and filters that are dye specific.

 

Digital

The digital types have become increasingly common, as increased functionality and ease of use make them very beneficial. Combined with operating the system using a PC or Mac, it simplifies data acquisition and analysis.

 

Phosphoimagers

This type enhances sensitivity for multiplex applications as well as radioisotopes. This, similarly to laser type, offers flexibility in choice regarding filters and includes customizable options.

 

We Offer Gel Documentation System Leases to Fit Every Need

a petri dish with a sample inside

Whatever your imaging or gel electrophoresis needs, we can help. We offer comprehensive lease options and sale-leasebacks as well. 

Furthermore, if you require protein purification instrumentation to prepare your samples for western blot or other imaging techniques, we can help with that as well. Our lease program includes a wide variety of nucleic acid and protein extraction/purification instruments and systems.

Contact us today at (510) 982-6552 or fill out our contact form to specify your equipment requirements.

Operating Lease

This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional monetary savings.

Sale-Leaseback

If you recently bought equipment, Excedr can offer you cash for your device and convert your purchase into a long-term rental. This is called a sale-leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up money in your budget rather than tying it down to a fixed asset.

Gel Imaging System Manufacturers & Models
Learn More

Thermo Fisher:
E-Gel Imager, iBright Imaging Systems, iBlot 2 Dry Blotting System, iBright CL750, iBright FL1000, iBright CL1000

Azure Biosystems:
Azure 300, Azure 400, Azure 500, Azure 600, Azure 280, Sapphire Biomolecular Imager, Azure 200

LI-COR:
C-DiGit Blot Scanner, Odyssey CLx Imaging System, Odyssey Fc Imaging System

Bio-Rad:
ChemiDoc MP Imaging System, ChemiDoc MP V3 Western Workflow, ChemiDoc Imaging System, Mini Trans-Blot Cell, Criterion Blotter, Trans-Blot Cell, Trans-Blot Plus Cell, V3 Western Workflow, ChemiDoc MP Imaging System, ChemiDoc System, Trans-Blot Turbo Transfer System, ChemiDoc XRS+System, GS-900 Calibrated Densitometer

UVItec Limited:
Alliance Q9 Mini, Alliance Q9 Advanced, Alliance Q9 Touch

Analytik Jena:
UVP ChemStudio Series, UVP ChemStudio PLUS, UVP ChemStudio, UVP ChemStudio touch, UPV ChemStudio Plus touch, UVP ChemStudio SA2, UVP ChemStudio PLUS Automated, UVP ChemStudio PLUS motorized

Syngene:
DigiGenius, InGenius3, NuGenius/NuGenius+, GeneGnome XRQ, G:BOX mini 6, G:BOX mini 9, G:BOX Chemi XX6, G:BOX Chemi XX9, G:Box Chemi XRQ

ProteinSimple:
FlourChem Systems, FlourChem E, FlourChem HD2, FlourChem FC3, FlourChem R, FlourChem M, , Simple Western Systems, Jess, Wes, Sally Sue, Peggy Sue, NanoPro 10000

Cytiva:
Amersham Imager 680, Amersham Typhoon, ImageQuant 800 systems, ImageQuant LAS 500, ImageQuant LAS 500 & IQTL, ImageQuant LAS 4000 Series

Vilber:
Fusion FX Spectra, Fusion FX, Fusion Solo S

NextAdvance:
BlotBot Blot Processor, Freedom Rocker BlotBot 120, Freedom Rocker BlotBot 240

GEL Company:
Omega Lum W Imaging System 81-12120-00, Omega Lum G Imaging System 81-12100-00, Omega Lum C Imaging System 81-12110-00, Omega Fluor Gel Documentation System, 365 nm 81-12530-00

and more!

 

Operating Capital Benefits

Operating Capital Benefits

Excedr's operating lease structure allows you to keep your business credit line open for expansions, staffing, and other operational expenses. Additionally, it strengthens the cash flow of your business and keeps cash reserves free for business development opportunities.

Effects on Credit

Effects on Credit

Leasing / renting provides you with non-dilutive financing and does not hinder your future borrowing ability. You're able to acquire the equipment you need without the baggage associated with traditional financing.

Used Equipment

Used Equipment

Unlike traditional financing and leasing companies, the Excedr program can accommodate refurbished/reconditioned equipment in addition to demo units. If you are looking for additional cost-savings, we recommend considering this option.

Speed of Approval

Speed of Approval

Excedr's program allows you to respond quickly as your need for equipment and technology arises. You can be approved with minimal documentation and have the equipment you need in operation and generating revenue for your business quickly.