PCR Systems:

PCR Systems

How PCR Systems Work & How We Save You Time & Money

Despite the diversity in methods, the Excedr lease program is able to source all instrument types and can accommodate any brand preferences your end-user might have. Request an estimate today and see how leasing can discount your PCR system or thermal cycler’s price.

All equipment brands/models are available

The Advantages of Excedr’s PCR System Leasing Program:

  • Eliminates the upfront cost of purchasing equipment by spreading its cost over time
  • Minimizes equipment downtime with included complete repair coverage and preventive maintenance
  • Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
  • Expedites the administrative work needed for instrument procurement and logistics
  • Conserves working capital,  enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
  • Accommodates all manufacturer and model preferences

*Please consult your tax advisor to determine the full tax implications of leasing equipment.

The polymerase chain reaction (PCR) is a technique used to amplify DNA sequences in vitro. It is widely used for many applications such as molecular biology, microbiology, genetics, pharmaceutical research, diagnostics, clinical laboratories, forensic science, environmental science, food science, hereditary studies, and paternity testing.

Despite revolutionizing scientific research, the principle of basic polymerase chain reaction is elegantly simple and involves a three-step reaction of thermal denaturation, primer annealing, and primer extension. PCR systems and thermal cyclers, also referred to as thermocyclers and PCR machines, facilitate this reaction and provide a thermally controlled environment for the PCR samples by changing the temperature of its heating block at specific times and by spending durations of time at specific temperatures. Once temperature cycling is complete, DNA amplification is confirmed using agarose gel electrophoresis, and although DNA can be quantified during endpoint analysis, it is generally considered more reliable to do this using real-time polymerase chain reaction.

 

Methods, Configurations, & PCR Machine Price

There are many variations of the original polymerase chain reaction method that have been developed over the years. We cover some of the most widely used in our PCR machine blog post, along with an explanation of how these devices operate, the cost of acquiring one, and the advantages of leasing vs. buying. Some polymerase chain reaction methods include:

Hot Start PCR
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Hot Start involves blocking DNA polymerase activity at low temperatures with specific anti-Taq antibodies and is used to reduce nonspecific priming/amplification and the creation of primer dimers. On top of that, it improves performance by increasing product yields. With Hot Start Long and Accurate, the impact on yield can be even more significant. Conventional methods, while effective, also involve additional handling and increased risk of contamination.

 

Reverse Transcriptase PCR
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The DNA polymerases used for basic PCR require a DNA template. There are, however, numerous instances in which amplification of RNA would be preferred. To study RNA, the RNA sample must first be reverse transcribed to cDNA using a reverse transcriptase enzyme. The resulting cDNA can then be used for subsequent polymerase chain reaction amplification. Reverse Transcriptase PCR (RT-PCR) is commonly used for gene expression profiling, inserting eukaryotic genes into prokaryotes, and disease diagnosis.

 

Quantitative Real-Time PCR
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Real-time PCR and RT-PCR enable accurate quantification of starting amounts of DNA, cDNA, and RNA targets. Real-time detection of polymerase chain reaction products is made possible by including in the reaction a fluorescent molecule that reports an increase in the amount of DNA with a proportional increase in fluorescent signal. Because it detects the amount of product as the reaction progresses, qPCR provides a wide linear dynamic range, demonstrates high sensitivity, and is very quantitative.

 

Real-Time PCR Detection System
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A specialized RT-PCR thermal cycler equipped with fluorescence detection modules is used to monitor the fluorescence as amplification occurs. When shopping for real-time PCR thermocyclers, considerations should include the footprint, multiplexing capabilities, compatibility with standard liquid handling workstations and multichannel pipettes, reagent and consumables cost, ease-of-use, software capabilities, and throughput potential. From an economic standpoint, we have seen a decrease in qPCR machine cost as technology has rapidly improved over the years, making these apparatuses much more accessible to the lab community.

 

Digital PCR Machine
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These devices provide absolute counts of target DNA, as well as increased sensitivity, precision, and reproducibility, compared to qPCR. Digital polymerase chain reaction has the potential to have a major impact on molecular analyses, ranging from clinical applications, such as biomarker analysis, viral detection, prognostic monitoring and fetal screening, to research applications such as phage-host interactions and intracellular profiling. Digital PCR can also be applied to assist with the library preparation needed for massively parallel next-generation sequencing methods. There are currently two types of dPCR platforms:

  • Droplet Digital PCR (ddPCR): Similar to qPCR, ddPCR utilizes Taq polymerase to amplify the targeted DNA in a complex sample. Additionally, it uses microfluidics to emulsify samples in oil, creating reproducible droplets to be processed and analyzed by fluorescence. Before being analyzed in the ddPCR process, a sample is divided into 20,000 droplets, undergoes thermal cycling, and then ran through a 96-well PCR plate. This partitioning of the sample into thousands of reaction vessels is a major difference between droplet digital PCR and qPCR. 
  • qdPCR: A platform based on integrated fluidic circuits (chips), sometimes abbreviated to qdPCR. Chip-based techniques have a narrower dynamic range; however, they provide more precise sample partitioning that can provide consistently high data collection with vastly reduced variance.

Both ddPCR and qdPCR approaches are at the forefront of detection technology, and accordingly, they are also the most expensive purchases one can opt for. As for the other aforementioned methods, they are relatively more affordable than dPCR but remain a large, burdensome capital expenditure.

 

Whether you’re looking to lease a qPCR system or leaseback a liquid handler, we can help you. At Excedr, your success is our goal. You can reach us at +1(510) 982-6552, or complete our contact form to specify your financing needs.

We Offer QPCR System Leases to Fit Every Need

Operating Lease

This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional monetary savings.

Sale-Leaseback

If you recently bought equipment, Excedr can offer you cash for your device and convert your purchase into a long-term rental. This is called a sale leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up money in your budget rather than tying it down to a fixed asset.


PCR Detection System Manufacturers & Models on the Market
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Thermo Fisher & Applied Biosystems: 
QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, QuantStudio 12k Flex, QuantStudio 3D, QuantStudio Dx, StepOne, StepOnePlus, ViiA 7, 7500 Fast, 7500 Fast Dx, 7500 Real-Time PCR System, PikoReal

Cole-Parmer: 
PCRmax

G-Storm: 
GS4822, GS1, GS2, GS4

Hygiena: 
BAX System X5, BAX System Q7

Fluidigm: 
Biomark HD, EP1

RainDance Technologies: 
RainDrop Plus Digital PCR System, ThunderBolts System

Eppendorf: 
MasterCycler

Bio-Rad: 
CFX96, CFX384, CFX Connect, QX200 Droplet Digital PCR System, QX200 AutoDG, ddSEQ

QIAGEN: 
Rotor-Gene Q

Agilent: 
AriaMx, AriaDx, Mx3005P, Mx3000P

Roche Life Science: 
LightCycler 96, LightCycler 480, LightCycler 1536, cobas LIAT, LightCycler 2.0

Analytik Jena: 
qTOWER

and more!

 


Operating Capital Benefits

Operating Capital Benefits

Excedr's operating lease structure allows you to keep your business credit line open for expansions, staffing, and other operational expenses. Additionally, it strengthens the cash flow of your business and keeps cash reserves free for business development opportunities.

Effects on Credit

Effects on Credit

Leasing / renting provides you with non-dilutive financing and does not hinder your future borrowing ability. You're able to acquire the equipment you need without the baggage associated with traditional financing.

Used Equipment

Used Equipment

Unlike traditional financing and leasing companies, the Excedr program can accommodate refurbished/reconditioned equipment in addition to demo units. If you are looking for additional cost-savings, we recommend considering this option.

Speed of Approval

Speed of Approval

Excedr's program allows you to respond quickly as your need for equipment and technology arises. You can be approved with minimal documentation and have the equipment you need in operation and generating revenue for your business quickly.