How Next-Generation Sequencing Works & How Our Services Save Time & Money
No matter what your equipment needs might be, Excedr’s lease program can procure virtually any equipment type and is able to accommodate countless brand preferences your end-user might have. Get in touch today and see how leasing can save you both time and money.
All equipment brands/models are available
The Benefits of Excedr’s NGS Leasing Program:
- Eliminates the upfront cost of purchasing equipment by spreading its cost over time
- Minimizes equipment downtime with included complete repair coverage and preventive maintenance
- Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
- Expedites the administrative work needed for instrument procurement and logistics
- Conserves working capital, enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
- Accommodates all manufacturer and model preferences
*Please consult your tax advisor to determine the full tax implications of leasing equipment.
When the Human Genome Project (HGP) began in 1990, the objective was to determine the DNA sequence of the entire euchromatic human genome within 15 years.
Many met the project with skepticism, as the huge costs of the project seemed to outweigh the potential benefits. Ultimately, it took researchers 10 years and $3B to complete the project.
However, it was widely considered a huge success, and ushered in a new era of medicine. The project’s completion also led to critical advances in the type of sequencing technology used to sequence DNA.
Several years later, the introduction of next-generation sequencing (NGS) brought a fundamentally different approach to DNA sequencing. It greatly reduced the cost and time it took to sequence an entire human genome, and, through advancements in the technology, allowed for targeted DNA enrichment methods that can perform even higher genome throughput at a reduced cost per sample.
What once took years and millions of dollars to complete now only takes a couple thousand dollars and a day or two, depending on the the platform and size of genome being sequenced.
Since its introduction, NGS technology has been used to determine the sequence of DNA or RNA (RNA-seq) to study genetic variation associated with diseases or other biological phenomena using DNA fragmentation (amplicon sequencing), library preparation, and sequencing, all at a massive scale. It continues to evolve at an unprecedented speed to this day.
Originally referred to as “massively parallel sequencing”, this high-throughput sequencing method allows scientists to:
- Routinely examine and sequence a single genome a large number of times (whole-genome sequencing)
- Observe individual changes in genes
- Study population variations in genes or biomarkers using whole exome sequencing
- Study the microbiome and metagenomics
- Differentiate cancer genomes from healthy genomes at higher rates of accuracy, sensitivity, and speed (making a significant impact on the field of oncology)
- Study the epigenome, providing comprehensive views of epigenetic modifications of many species and cell types.
- Investigate the possibility of personalized medicine
In addition, the development of RNA-seq is due in large part to the success of NGS. It has increased scientists’ and researchers’ understanding of RNA biology, gene expression, and the transcriptome, thanks to innovations in long-read and direct RNA-seq technologies as well as advanced computational tools for data analysis.
Furthermore, it allows for de novo sequencing and assembly, which is used when sequencing a novel genome without a reference sequence available for alignment.
Next-Generation Sequencer Methods, Profiling, Applications, & More
Regardless of the specific method, next-generation sequencers rely on the generation of representative, unbiased sources of nucleic acid templates from the complex genomes being interrogated. Clonal amplification of DNA templates, or single DNA molecules, are sequenced in a massively parallel fashion in a flow cell.
The sequencing is conducted in either a stepwise iterative process or in a continuous real-time manner. In this way, the instruments allow for the sequencing of up to billions of individual DNA templates in a single reaction.
There are several main suppliers of NGS platforms and second generation sequencing platforms. This includes Illumina, Thermo Fisher Scientific, Oxford Nanopore Technologies, Roche, and Agilent, among several others. They all share the same fundamental sequencing process, but with varying technologies.
Let’s take a look at the various methods and technologies being used today.
Single-Molecule, Real-Time Technology
Sequencing by Synthesis Technology
NGS vs. Sanger Sequencing
Bioinformatics & Clinical Applications
Next-Generation Sequencing Technologies for Lease
If you’re interested in purchasing a next-generation sequencer but wish to avoid spending an enormous amount of capital upfront, consider leasing NGS technologies instead.
Leasing your cutting-edge lab equipment can not provide access to top-of-the-line instrumentation, it can also help you manage cash flow more easily and save room in the budget for other important areas, such as hiring more talented people or buying expensive yet necessary consumables.
Additionally, if you’re considering integrating cell sorting technologies and want to lease a flow cytometer (and use it in conjunction with your sequencer), we can help.
Reach us at +1 888-927-3802 or fill out our contact form and we can discuss your specific needs in detail.
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional monetary savings.
If you recently bought equipment, Excedr can offer you cash for your device and convert your purchase into a long-term rental. This is called a sale-leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up money in your budget rather than tying it down to a fixed asset.