How Gel Electrophoresis Works & How We Save You Time & Money
Despite the diversity in applications and methods, our lease program is able to source any instrument type for your laboratory, and can accommodate whichever brand preferences you or your end-user might have. Request a lease estimate today and learn more about the benefits of leasing your next gel electrophoresis instrument or chamber.
All equipment brands/models are available
The Advantages of Excedr’s Gel Electrophoresis System Leasing Program:
- Eliminates the upfront cost of purchasing equipment by spreading its cost over time
- Minimizes equipment downtime with included complete repair coverage and preventive maintenance
- Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
- Expedites the administrative work needed for instrument procurement and logistics
- Conserves working capital, enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
- Accommodates all manufacturer and model preferences
*Please consult your tax advisor to determine the full tax implications of leasing equipment.
Electrophoresis is used in the life sciences and biotechnology to separate and extract DNA fragments, RNA, and proteins, based on their size and charge, in order to analyze them.
It is used as a preparative technique in laboratories for PCR, Southern blotting, genome mapping, and DNA sequencing, and is also used in DNA fingerprinting and plant breeding. Scientists studying molecular biology, microbiology, and biochemistry utilize electrophoresis for studies and experiments in medicine, forensic science, and conservation biology, just to name a few.
Gel electrophoresis is one of the most common electrophoresis methods used.
In these systems, molecular samples are loaded into the electrophoresis apparatus, a type of box or chamber referred to as a gel box. The gel box is filled with an agarose or polyacrylamide gel and is equipped with a gel comb, as well as a negative electrode, or anode, on one side, and a positive electrode, or cathode, on the other. The comb helps form the wells where the samples are placed, and the negative and positive electrodes create an electrical current that generates electrophoretic separation.
The electrical current generated separates the DNA fragments based on their size or charge because, even though DNA molecules have the same amount of charge per mass, longer DNA fragments tend to have a higher molecular weight compared to shorter DNA pieces. That difference means segments move at varying speeds through the gel medium, grouping up according to size.
After gel electrophoresis is performed, the gel is stained with a fluorescent binding dye, which eventually allows the groups that formed to be more easily observed. Having stained the gel, a light source is then used to excite the fluorescent dye so that the DNA bands are visible for post-separation and analysis.
A well-defined group or line of DNA is referred to as a separation band. These separation bands tell us more about the DNA fragments size or charge.
Gel Types, Techniques, Buffers, & Automation
There are different types of gel used for different tasks, and the amount used can vary depending on the size of macromolecules in the sample.
The gel interacts with a buffer that affects the porousness of the gel matrix. Depending on the application, you’ll want to use a certain gel.
Nucleic Acid Electrophoresis
Gel Conditions Explained
Gel Documentation System Leases to Fit Every Need
Electrophoresis is a commonly used preparative technique essential in any biotechnology company that’s focused on molecular biology, microbiology, or biochemistry.
Worried about the upfront costs of gel electrophoresis equipment, or any other analytical separation technique, such as liquid chromatography or western blotting? Leasing with Excedr might be the option for you.
Spreading out the cost of an acquisition can help you keep daily operations going, and with our preventative maintenance and repair coverage, you can have peace of mind that any instrument downtime is being actively minimized.
We offer leases that can be customized to your financial and operational needs. Let us know how we can help and request a lease estimate today.
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional monetary savings.
If you recently bought equipment, Excedr can offer you cash for your device and convert your purchase into a long-term rental. This is called a sale-leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up money in your budget rather than tying it down to a fixed asset.