How Flow Cytometers Work & How We Save You Time & Money
Excedr’s lease program can accommodate your lab’s instrument needs and brand/model preferences. Get in touch with us today and see how leasing can save you time and money procuring flow cytometry technology.
All equipment brands/models are available
The Advantages of Our Flow Cytometer Leasing Program:
- Eliminates the upfront cost of purchasing equipment by spreading its cost over time
- Minimizes equipment downtime with included complete repair coverage and preventive maintenance
- Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
- Expedites the administrative work needed for instrument procurement and logistics
- Conserves working capital, enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
- Accommodates all manufacturer and model preferences
*Please consult your tax advisor to determine the full tax implications of leasing equipment.
Flow cytometry is a widely used method for measuring cellular properties as they move through a fluid stream past an optical system comprising an excitation light source (laser), lenses, mirrors, and lastly, filters, which are placed in front of detectors.
The cellular characteristics measured include the expression of cell surface and intracellular molecules, various cell types in heterogeneous cell populations, purity of isolated subpopulations, and single-cell size and volume. The resulting emitted photons are collected and become the data that identifies characteristics about the cells in question. It is a powerful technique commonly used in the life sciences.
This technique is also often used for high-throughput measurement of antigens on live cells. There is no better option in this case, as the technique utilizes fluorescent markers on antibodies or other proteins in order to quantifiably detect changes in protein expression via excitation of the various fluorescent markers. Antibodies allow scientists to detect a specific antigen, making them useful tools for protein characterization on the surface of live cells.
The antibodies can be directly labeled with a fluorescent marker—such as FITC (fluorescein isothiocyanate)—or can be visualized by binding to a secondary antibody that is labeled. This allows flow cytometry to analyze or sort cells based on more than one color, each representing a different antigen that is bound by a different antibody.
Over the past 30 years, single-cell flow cytometry analysis has become an indispensable tool for many scientific researchers and clinicians working in the life sciences performing data analysis and, more specifically, cell analysis.
The evolving technology ensures its application in a number of fields, including cell biology, drug discovery, cancer research, neuroscience, stem cell research, pathology, immunology, hematology, plant biology, food science, and marine biology.
FCM Components, Data Visualization, Sample Handling, & More
Flow cytometers, sometimes referred to as cell sorters (depending on whether cell analysis or cell sorting is being performed), are made up of three main components that are integral to collecting accurate flow cytometry data.
The fluidics system transports particles in a sample stream—with the help of flow cells—to the interrogation point and takes away the waste. Flow cells are sample cells designed to help liquid samples continuously flow through the beam path. This is especially useful for any samples that can be easily damaged by the excitation light source.
The optics system consists of a series of optical filters and mirrors to direct light of specific wavelengths emitted by cells or particles towards the detectors, amplifying the signal produce by single cells.
The electronics system then converts the signals from the detectors into electronic signals that can be processed by the computer. For some instruments equipped with a sorting feature, the electronics system is also capable of initiating sorting decisions to charge and deflect cells or particles. This is often referred to as fluorescence-activated cell sorting, or FACS.
The Fluidics System
The Optics System
The Electronics System
We Lease a Variety of FACS Machine Brands & Models
Regardless of whether you’re looking for microscopy or flow cytometry solutions, we’re here to help.
Excedr offers leases on flow cytometer models such as the cytoFLEX, s3e cell sorter, Attune NxT, and the entire MACSQuant line. Furthermore, by leasing with Excedr, you can acquire the exact instrument you need and keep more cash on hand for other critical areas, like hiring scientists and buying consumables.
If you don’t see your preferred instrument listed, we still may be able to help. Specify your exact leasing needs by completing our contact form or callling us at +1 888-927-3802 and we’ll be in touch.
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional monetary savings.
If you recently bought equipment, Excedr can offer you cash for your device and convert your purchase into a long-term rental. This is called a sale-leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up money in your budget rather than tying it down to a fixed asset.