How Bio-Layer Interferometers Work & How We Save You Time & Money
Regardless of configuration, the Excedr lease program can accommodate your lab’s instrument needs and model preferences. Get in touch with us to learn how leasing can save you time and money on your bio-layer interferometry technology.
All equipment brands/models are available
The Advantages of Excedr’s Biolayer Interferometer Leasing Program:
- Eliminates the upfront cost of purchasing equipment by spreading its cost over time
- Minimizes equipment downtime with included complete repair coverage and preventive maintenance
- Takes advantage of potentially 100% tax deductible* payments, providing you significant cash-savings
- Expedites the administrative work needed for instrument procurement and logistics
- Conserves working capital, enabling you to reinvest in your core business and operations (staffing, inventory, marketing/sales, etc.)
- Accommodates all manufacturer and model preferences
*Please consult your tax advisor to determine the full tax implications of leasing equipment.
Bio-layer interferometry, or BLI, is an optical analytical technique that observes the associative and dissociative interaction of molecules.
In chemistry, it is important to know the affinity, thermodynamic properties, and stoichiometry of a substance, or analyte. BLI provides this data by observing and measuring biomolecular interactions, particularly protein interactions, in vitro. It is an important tool in a wide variety of fields.
Using white light, BLI looks at how molecules bind to or disengage from other molecules. Because it is also a label-free technique, it does not use any isotopes that would chemically bind to and label proteins. These features make BLI highly useful for applications such as fragment screening, kinetic characterization, and real-time label-free chemical binding measurements. It is considered a strong alternative to Surface Plasmon Resonance (SPR), however, in comparison, it does have its advantages and disadvantages.
Bio-Layer Interferometer Applications, Benefits, & Costs
In order to measure biomolecular interactions, BLI shines white light through a biosensor tip. In many cases, a Streptavidin (SA) biosensor is used. However, the biosensor tips available are customizable and vary in use based on the molecule being immobilized and characterized.
The sensor tip has two surfaces inside of it: an internal reference layer and a layer of immobilized proteins on the sensor tip. As the proteins interact with the sample, some molecules will bind to them. Molecular immobilization occurs through the utilization of the high affinity streptavidin-biotin interaction. The biological molecules—in this case, proteins—can be biotinylated using established protocols and immobilized onto the biosensor surface for assay development. In other words, biotin is attached to the protein in order to make detection of the protein possible. The high sensitivity and stable baseline of these biosensor tips supports both kinetic and quantitation experiments.
The more molecules—or ligands—that bind to the immobilized protein, the more interference will occur with the light as it interacts with it. The resulting reflected light’s wavelength will then be shifted. This shifted wavelength is then compared to the reference wavelength. The molecules that are bound or dissociating from the biosensor are the source of the interference pattern.
Other factors such as unbound molecules, changes in flow rate, and the refractive index of the surrounding medium do not have the same effect. Because of this, the resulting data from BLI can give great insight into a substance’s kinetics, quantitation, affinity, and molecular interactions.
Let’s look at some of the applications and benefits BLI offers.
Study of Kinetics
Analysis of Protein/Liposome Interactions
Analysis of Protein/Protein Interactions
We Offer BLI Leases to Fit Every Need
If your lab studies—or plans to study—biochemistry or cell biology, Excedr can lease BLI technology or Surface Plasmon Resonance (SPR) instrumentation. Get the best data analysis possible, save money on upfront costs, and protect your equipment with comprehensive service plans.
Discuss your exact equipment needs in-depth with us by calling +1 888-927-3802 or completing our contact form.
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional monetary savings.
If you recently bought equipment, Excedr can offer you cash for your device and convert your purchase into a long-term rental. This is called a sale leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up money in your budget rather than tying it down to a fixed asset.