Protein Purification & Isolation

three syringes and two test tubes all containing samples of proteins

Overview & Resources

Proteins play an integral role in cell structure and function. In biochemistry, a protein is a polymer of amino acids arranged in the form of polypeptide chains. In order to study proteins, it’s important to first identify and isolate them. To accomplish this, biochemists carry out high-throughput protein purification.

First, individual proteins are isolated. Then, they are separated based on molecular weight, solubility, charge, and specific binding affinity. The multi-step process helps scientists and researchers analyze individual proteins and study protein characterization and interactions. Protein purification is carried out in order to obtain and study the desired protein from a complex mixture.

Isolating a protein will help in the analysis of that protein’s:

  • Size and structure
  • Binding affinity
  • Biological activity
  • Physio-chemical properties
  • Protein-protein interactions

The process of protein purification and isolation holds many experimental applications, especially in the large-scale production of recombinant proteins.

Learn more about protein purification and isolation, its applications, and the consumables used to make this technique happen below.

Protein Purification & Isolation Resources

Lectin: Application & Overview

Lectin: Application & Overview

Lectin: Overview Lectins are carbohydrate-binding proteins that recognize and reversibly bind the carbohydrate moieties of complex glycoconjugates. Their molecular interaction with sugar groups does n…
Biotinylation: Application & Overview

Biotinylation: Application & Overview

Biotinylation: Overview Fusion tags are widely used in labs for the identification and isolation of recombinant proteins that are expressed in bacteria or other cells (such as E.coli, plants, or mamma…
What Is Avidin: Overview, Application, & Industry Uses

What Is Avidin: Overview, Application, & Industry Uses

Naturally occurring interactions have been a great tool in a spectrum of labs, ranging from biotechnology and applied physics to medicine and biochemistry to achieve countless scientific milestones. O…
How Does Streptavidin Work? Function, Structure, & Uses

How Does Streptavidin Work? Function, Structure, & Uses

Streptavidin: Overview Streptavidin is a tetrameric protein isolated from the bacterium Streptomyces avidinii. It’s highly known for its lesser non-specific binding with the biotin (or Vitamin H) mole…
Immunoprecipitation: Applications, Uses, & Types

Immunoprecipitation: Applications, Uses, & Types

Immunoprecipitation is a small-scale affinity purification technique to enrich or isolate a protein from biological samples, such as tissue or cell lysate. It works on the principle of antigen-antibod…
What Are Protein A Magnetic Beads?

What Are Protein A Magnetic Beads?

Application & Overview Protein A magnetic beads are an affinity matrix for the purification and isolation of antibodies or immunoglobulins. The beads are formed by covalently linking the truncated…
FLAG Tag: Application & Overview

FLAG Tag: Application & Overview

FLAG TAG: Overview The FLAG tag is a protein tag widely used in protein purification procedures for the high specificity and purity of target proteins. When high purity is required, it is commonly use…
Monoclonal Antibodies: Overview & Applications

Monoclonal Antibodies: Overview & Applications

What are Monoclonal Antibodies? Monoclonal antibodies are proteins developed in labs from the clone of a single B-cell. They possess an affinity for a specific antigen and are produced in large quanti…
Ion Exchange Resins: What They Are and Their Applications

Ion Exchange Resins: What They Are and Their Applications

Definition: Ion Exchange Resins Ion exchange resins are insoluble polymers formed by crosslinking of polystyrenes with different functional groups, which act as a medium for ion exchange reactions.&nb…
Protein A Resin: Definition & Overview

Protein A Resin: Definition & Overview

Definition: Protein A Resin Protein A resin is a high-affinity chromatography medium. It’s used for the one-step purification of monoclonal antibody products (mAb) and immunoglobulins from a cell cult…

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