Protein Purification & Isolation

three syringes and two test tubes all containing samples of proteins

Overview & Resources

Proteins play an integral role in cell structure and function. In biochemistry, a protein is a polymer of amino acids arranged in the form of polypeptide chains. In order to study proteins, it’s important to first identify and isolate them. To accomplish this, biochemists carry out high-throughput protein purification.

First, individual proteins are isolated. Then, they are separated based on molecular weight, solubility, charge, and specific binding affinity. The multi-step process helps scientists and researchers analyze individual proteins and study protein characterization and interactions. Protein purification is carried out in order to obtain and study the desired protein from a complex mixture.

Isolating a protein will help in the analysis of that protein’s:

  • Size and structure
  • Binding affinity
  • Biological activity
  • Physio-chemical properties
  • Protein-protein interactions

The process of protein purification and isolation holds many experimental applications, especially in the large-scale production of recombinant proteins.

Learn more about protein purification and isolation, its applications, and the consumables used to make this technique happen below.

Protein Purification & Isolation Resources

FLAG Tag: Application & Overview

FLAG Tag: Application & Overview

FLAG TAG: Overview The FLAG tag is a protein tag widely used in protein purification procedures for the high specificity and purity of target proteins. When high purity is required, it is commonly use…
Monoclonal Antibodies: Overview & Applications

Monoclonal Antibodies: Overview & Applications

What are Monoclonal Antibodies? Monoclonal antibodies are proteins developed in labs from the clone of a single B-cell. They possess an affinity for a specific antigen and are produced in large quanti…
Ion Exchange Resins: What They Are and Their Applications

Ion Exchange Resins: What They Are and Their Applications

Definition: Ion Exchange Resins Ion exchange resins are insoluble polymers formed by crosslinking of polystyrenes with different functional groups, which act as a medium for ion exchange reactions.&nb…
Protein A Resin: Definition & Overview

Protein A Resin: Definition & Overview

Definition: Protein A Resin Protein A resin is a high-affinity chromatography medium. It’s used for the one-step purification of monoclonal antibody products (mAb) and immunoglobulins from a cell cult…
What is a Protein Ladder or Protein Marker?

What is a Protein Ladder or Protein Marker?

Protein ladders are used in experiments to estimate the size of proteins of interest and are resolved using gel electrophoresis. They are also known as protein markers, protein standards, or protein m…

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