Nucleic Acid & Protein Purification & Isolation

Overview & Resources

DNA and proteins play an integral role in cell structure and function. In biochemistry, a protein is a polymer of amino acids arranged in the form of polypeptide chains. In order to study proteins, it’s important to first identify and isolate them. To accomplish this, biochemists carry out high-throughput protein purification.

First, individual proteins are isolated. Then, they are separated based on molecular weight, solubility, charge, and specific binding affinity. The multi-step process helps scientists and researchers analyze individual proteins and study protein characterization and interactions. Protein purification is carried out in order to obtain and study the desired protein from a complex mixture.

Isolating a protein will help in the analysis of that protein’s:

• Size and structure
• Binding affinity
• Biological activity
• Physio-chemical properties
• Protein-protein interactions

The process of protein purification and isolation holds many experimental applications, especially in the large-scale production of recombinant proteins.

Learn more about protein purification and isolation, its applications, and the consumables used to make this technique happen below.