How Fluorescence Microscopy Works & How We Save You Time & Money
Here are the advantages of Excedr’s fluorescence microscope leasing program:
- We enable you to spread out payments over time and remove the large upfront cost of equipment purchases
- Payments may be 100% tax deductible*, which makes leasing even more low-cost
- Purchase equipment outright is more expensive than our leasing
- Reduction of equipment downtime through our comprehensive repair coverage
- We deal with and expedite the administrative work needed for instrument procurement and maintenance
- The money saved with our leasing program can be reinvested in your core business and operations (staffing, inventory, marketing/sales, etc.)
*Please consult your tax advisor to determine the full tax implications of leasing equipment.
All equipment brands/models are available
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Fluorescence refers to the emission of light due to the absorption of light. When a fluorescent material is exposed to electromagnetic radiation, it absorbs that radiation and re-emits some of it. The time difference, less than a millisecond, between when the organic or inorganic object absorbs the radiation and emits it is short due to the photons’ inherent absorption and emission time. The object will stop fluorescing once it stops being hit by radiation. When the materials continue to emit light after the source of the radiation has gone it is called phosphorescence. The re-emitted light from fluorescent objects has lower energy and thus a lower wavelength signature, making it exploitable in different analytical techniques. An example of fluorescence can be seen when one uses a blacklight and previously unseen colors suddenly are illuminated. Other common examples of fluorescences are minerals and various other bioluminescent animals and plants like algae, fish, or insects.
A fluorescence microscope is a form of optical microscope that exploits fluorescence and phosphorescence to identify and observe specific microscopic objects. The area of the material that is to be observed is dyed with fluorophore tags that will illuminate only the intended parts of the sample. The specimen is struck with electromagnetic radiation of a specific wavelength, which is absorbed by the fluorophores which then fluoresce radiation with longer wavelengths than the incident light. This difference in wavelengths can then be used to filter out the light from the light source and only analyze light from the fluorescent sample by using a spectral emission filter. The main components that make up fluorescence microscopes include:
- Light source: Common types are xenon arc lamps and mercury vapor lamps
- Excitation filter: Filters out undesirable wavelengths of light from light source.
- Dichroic mirror: Filter out specific spectra of light or colors. Also called interference filters or accurate color filters.
- Emission filter: Filter out non-fluorescent light spectra so only desired fluorescence is analyzed.
Different specific filters and beam splitters are chosen depending on the specific fluorophore used to label the specimen. Single fluorophores are imaged at one time meaning that a narrow wavelength or one “color” that is emitted from the sample are observed at one time. Multi-colored images can be composed by sewing multiple one color images together.
Fluorophore Imaging Types, Methods, & Cost
The most widely used type of fluorescent microscope is epifluorescence or epi-illumination microscopes. Most microscopes have light travel from the light source, through the sample, and into the objective lens where the image magnification occurs. This means the light sources shines from beneath the sample. In epifluorescence microscopy, the specimen is illuminated from above. This means that the emitted fluorescence and the illuminating light both travel through the same objective lens. Due to the excitation light traveling through the objective lens to get to the sample and not the other way around, less light needs to be filtered out to isolate the fluorescent light.
Total Internal Reflection (TIRF)
A problem that can occur in fluorescence microscopy is that in thicker objects, the light fluorescing from the background matter will drown out the light fluorescing from the surface of the object. TIRF microscopes are used to focus on just these surface-bound-fluorophores’ emitted light. This is done by using evanescent waves for excitation of the fluorophores rather than using direct light from a lamp. Evanescent fields or waves are spatially concentrated in the area that they occur. This means that when these waves occur they only excite the fluorophores on the surface of the sample. TIRF’s sub-micron surface selectivity make it the main method for single molecule detection.
For a long time, most of the scientific community accepted the fact that optical microscopy would never be used to observe nano-dimensional objects. The conventional wisdom was that the limitations on the resolution of wavelengths that can be obtained would limit the usefulness of optical microscopy. The 2014 Nobel Laureates in Chemistry, however, have completely changed this. Eric Betzig, William Moerner, and Stefan Hell were all awarded the Nobel Prize in Chemistry “for the development of super-resolved fluorescence microscopy.” Their discoveries have brought optical microscopy into the nanodimension.
In 2000, Stefan Hell developed stimulated emission depletion (STED) microscopy, a technique that utilizes two lasers to achieve nano-level microscopy. One laser is used to fluoresce specific molecules, while the second one is used to cancel out undesired wavelengths. Eric Betzig and William Moerner developed single-molecule microscopy which allows them to turn molecules fluorescent qualities on and off again. Using multiple images with different molecules either on or off, they are able to also achieve nano-level microscopy. These are revolutionary techniques that are now allowing scientists to take a better look at the world around us. These nanoscopy techniques are now being used to look at synaptic behavior between nerve cells in the brain to better understand diseases like Alzheimer’s, Parkinson’s, and Huntington’s disease.
Whatever your need, a fluorescence microscope can be a vital part of any lab and acquiring one should not financially hinder you. Excedr’s fluorescence microscope leasing can allow you to responsibly finance your microscope while avoiding the burdensome cost of purchasing it outright. Let us take care of your repair and preventative maintenance needs while your lab focuses on its important work.
We Offer TIRF Fluorescence Microscope Leases to Fit Every Need
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional monetary savings.
If you recently bought equipment, Excedr can offer you cash for your device and convert your purchase into a long-term rental. This is called a sale leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up money in your budget rather than tying it down to a fixed asset.
EPIFLUORESCENCE MICROSCOPE MANUFACTURERS & MODELS ON THE MARKET
- ThermoFisher: EVOS Series, EVOS M5000, EVOS M7000, EVOS XL, EVOS XL Core, EVOS FL, EVOS FL Auto, EVOS FL Auto 2, EVOS FLoid, Cellnsight CX7, Cellnsight CX5
- Zeiss: Axio Zoom.V16, Axio Scan.Z1, Celldiscoverer 7, Lightsheet Z.1, Apotome.2
- Olympus: MVX10
- Leica: TCS SP8 STED ONE, TCS SP8 STED 3X, M205 FCA, M205 FA, M165 FC, MZ10 F, FluoCombi III, TCS SP8 STED
- BioTek: Lionheart LX, Cytation 1
- Bruker: Vutara 352
- Luxendo: Light-SHeet: InVi SPIM, QuVi SPIM, MuVi SPIM
- Keyence: BZ-X Series, BZ-X800
- Inova Diagnostics: NOVA View
- Molecular Devices: ImageXpress Nano
- Fraen Corporation: FLUOLED Series, FLUOLED TB-SCOPE
- Auxilab: Epi-fluorescence microscope 158/358
- MSHOT: MF43, MZX11, MZX81, MF53, MF52, MF10-LED, MF31
- Euromex iScope, Delphi-X, Oxion, Oxion Inverso, bScope, B+ series, DZ series
- LW Scientific: Innovation Lumin, i4 Lumin, Mi5 Lumin
- View Solutions Inc.
- TauTec: TauScope TD
- and more!
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Effect on Credit & Operating Capital
Leasing/renting does not hinder your future borrowing ability and allows you to keep your business credit line open for expansions, staffing, and other operational expenses. Additionally, it strengthens the cash flow of your business and keeps cash reserves free for business development opportunities.
Unlike traditional financing and leasing companies, the Excedr program can accommodate refurbished/reconditioned equipment in addition to demo units. If you are looking for additional cost-savings, we recommend considering this option.
Speed of Approval
Excedr’s program allows you to respond quickly as your need for equipment and technology arises. You can be approved with minimal documentation and have the equipment you need in operation and generating revenue for your business quickly.